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A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice

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Abstract

Rice blast disease caused by Magnaporthe grisea is a continuous threat to stable rice production worldwide. In a modernized agricultural system, the development of varieties with broad-spectrum and durable resistance to blast disease is essential for increased rice production and sustainability. In this study, a new gene is identified in the introgression line IR65482-4-136-2-2 that has inherited the resistance gene from an EE genome wild Oryza species, O. australiensis (Acc. 100882). Genetic and molecular analysis localized a major resistance gene, Pi40(t), on the short arm of chromosome 6, where four blast resistance genes (Piz, Piz-5, Piz-t, and Pi9) were also identified, flanked by the markers S2539 and RM3330. Through e-Landing, 14 BAC/PAC clones within the 1.81-Mb equivalent virtual contig were identified on Rice Pseudomolecule3. Highly stringent primer sets designed for 6 NBS-LRR motifs located within PAC clone P0649C11 facilitated high-resolution mapping of the new resistance gene, Pi40(t). Following association analysis and detailed haplotyping approaches, a DNA marker, 9871.T7E2b, was identified to be linked to the Pi40(t) gene at the 70 Kb chromosomal region, and differentiated the Pi40(t) gene from the LTH monogenic differential lines possessing genes Piz, Piz-5, Piz-t, and Pi-9. Pi40(t) was validated using the most virulent isolates of Korea as well as the Philippines, suggesting a broad spectrum for the resistance gene. Marker-assisted selection (MAS) and pathotyping of BC progenies having two japonica cultivar genetic backgrounds further supported the potential of the resistance gene in rice breeding. Our study based on new gene identification strategies provides insight into novel genetic resources for blast resistance as well as future studies on cloning and functional analysis of a blast resistance gene useful for rice improvement.

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Acknowledgments

We are grateful to the Rural Development Administration (RDA), Suwon, Korea, for financial support for this study. We thank Darshan S. Brar (plant breeder, IRRI) and Guo-Liang Wang, Ohio State University, USA for critical review of the manuscript, Y. Fukuta (plant breeder, IRRI) for providing blast monogenic differential lines and C. M. Veracruz (plant pathologist, IRRI) for evaluating the lines against Philippines blast isolates. We are thankful to Ms. Kang Min-Hae for technical assistance and Bill Hardy (science editor, IRRI) for editing the manuscript.

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Authors and Affiliations

  1. Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, Los Baños, Laguna, Philippines

    J. U. Jeung & K. K. Jena

  2. IRRI-Korea Office, National Institute of Crop Science, RDA, Suwon, 441-857, Republic of Korea

    J. U. Jeung & K. K. Jena

  3. Genetics and Breeding Division, National Institute of Crop Science, RDA, Suwon, 441-857, Republic of Korea

    J. U. Jeung, Y. C. Cho, H. P. Moon & Y. T. Lee

  4. Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA, Suwon, 441-707, Republic of Korea

    B. R. Kim & S. S. Han

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  1. J. U. Jeung
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  7. K. K. Jena
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Correspondence to K. K. Jena.

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Communicated by T. Sasaki

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Jeung, J.U., Kim, B.R., Cho, Y.C. et al. A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice. Theor Appl Genet 115, 1163–1177 (2007). https://doi.org/10.1007/s00122-007-0642-x

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