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Functional analysis of Xa3/Xa26 family members in rice resistance to Xanthomonas oryzae pv. oryzae

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Abstract

Plant disease resistant (R) genes are frequently clustered in the genome. The diversity of members in a complex R-gene family may provide variation in resistance specificity. Rice Xa3/Xa26, conferring resistance to Xanthomonas oryzae pv. oryzae (Xoo) encodes a leucine-rich repeat (LRR) receptor kinase-type protein and belongs to a multigene family, consisting of Xa3/Xa26, MRKa, MRKc and MRKd in rice cultivar Minghui 63. MRKa and MRKc are intact genes, while MRKd is a pseudogene. Complementary analyses showed that MRKa and MRKc could not mediate resistance to Xoo when regulated by their native promoters, but MRKa not MRKc conferred partial resistance to Xoo when regulated by a strong constitutive promoter. Plants carrying truncated XA3/XA26, which lacked the kinase domain, were compromised in their resistance to Xoo. However, the kinase domain of MRKa could partially restore the function of the truncated XA3/XA26 in resistance. MRKa and MRKc showed similar expression pattern as Xa3/Xa26, which expressed only in the vascular systems of different tissues. The expressional characteristic of MRKa and MRKc perfectly fits the function of genes conferring resistance to Xoo, a vascular pathogen. These results suggest that although MRKa and MRKc cannot mediate bacterial blight resistance nowadays, they may be once effective genes for Xoo resistance. Their expressional characteristic and sequence similarity to Xa3/Xa26 will provide templates for generating novel recognition specificity to face the evolution of Xoo. In addition, both LRR and kinase domains encoded by Xa3/Xa26 and MRKa are the functional determinants and MRKa-mediated resistance is dosage-dependent.

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Acknowledgments

This work was supported by grants from the National Program on the Development of Basic Research in China, the National Program of High Technology Development of China, and the National Natural Science Foundation of China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China

    Yinglong Cao, Liu Duan, Hongjing Li, Xinli Sun, Yu Zhao, Caiguo Xu, Xianghua Li & Shiping Wang

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  1. Yinglong Cao
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  2. Liu Duan
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  3. Hongjing Li
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  4. Xinli Sun
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  5. Yu Zhao
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  6. Caiguo Xu
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  7. Xianghua Li
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  8. Shiping Wang
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Correspondence to Shiping Wang.

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Communicated by M. Xu.

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Cao, Y., Duan, L., Li, H. et al. Functional analysis of Xa3/Xa26 family members in rice resistance to Xanthomonas oryzae pv. oryzae . Theor Appl Genet 115, 887–895 (2007). https://doi.org/10.1007/s00122-007-0615-0

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  • DOI: https://doi.org/10.1007/s00122-007-0615-0

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