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The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante

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Abstract

Verticillium wilt of lettuce caused by Verticillium dahliae can cause severe economic damage to lettuce producers. Complete resistance to race 1 isolates is available in Lactuca sativa cultivar (cv.) La Brillante and understanding the genetic basis of this resistance will aid development of new resistant cultivars. F1 and F2 families from crosses between La Brillante and three iceberg cultivars as well as a recombinant inbred line population derived from L. sativa cv. Salinas 88 × La Brillante were evaluated for disease incidence and disease severity in replicated greenhouse and field experiments. One hundred and six molecular markers were used to generate a genetic map from Salinas 88 × La Brillante and for detection of quantitative trait loci. Segregation was consistent with a single dominant gene of major effect which we are naming Verticillium resistance 1 (Vr1). The gene described large portions of the phenotypic variance (R 2 = 0.49–0.68) and was mapped to linkage group 9 coincident with an expressed sequence tag marker (QGD8I16.yg.ab1) that has sequence similarity with the Ve gene that confers resistance to V. dahliae race 1 in tomato. The simple inheritance of resistance indicates that breeding procedures designed for single genes will be applicable for developing resistant cultivars. QGD8I16.yg.ab1 is a good candidate for functional analysis and development of markers suitable for marker-assisted selection.

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References

  • Alexander LJ (1962) Susceptibility of certain Verticillium-resistant tomato varieties to an Ohio isolate of the pathogen. Phytopathology 52:998–1000

    Google Scholar 

  • Anonymous (2005) Monterey County crop report 2004. Monterey County Agricultural Commissioner’s Office, Salinas, CA

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DL (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Atallah ZK, Maruthachalam K, du Toit L, Koike ST, Davis RM, Klosterman SJ, Hayes RJ, Subbarao KV (2010) Population analyses of the vascular plant pathogen Verticillium dahliae detect recombination and transcontinental gene flow. Fungal Genet Biol 47:416–422

    Article  PubMed  CAS  Google Scholar 

  • Bae J, Halterman D, Jansky H (2008) Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific hybrids. Mol Breed 22:61–69

    Article  CAS  Google Scholar 

  • Diwan N, Fluhr R, Eshed Y, Zamir D, Tanksley SD (1999) Mapping of Ve in tomato: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1. Theor Appl Genet 98:315–319

    Article  CAS  Google Scholar 

  • Fehr W (1991) Principles of cultivar development, v. 1. Theory and technique. Walter R Fehr, USA

    Google Scholar 

  • Fradin EF, Thomma BPHJ (2006) Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol Plant Pathol 7:71–86

    Article  PubMed  CAS  Google Scholar 

  • Fradin EF, Zhang Z, Juarez Ayala JC, Castroverde CDM, Nazar RN, Robb J, Liu C-M, Thomma BPHJ (2009) Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1. Plant Physiol 150:320–332

    Article  PubMed  CAS  Google Scholar 

  • Garibaldi A, Gilardi G, Gullino ML (2007) First report of Verticillium wilt caused by Verticillium dahliae on lettuce in Italy. Plant Dis 91:770

    Article  Google Scholar 

  • Hartnett JP, Lorbeer JW (1971) The production of a noninfectious lettuce root rot under controlled environmental and soil conditions. Phytopathology 61:1153–1158

    Article  Google Scholar 

  • Hayes RJ, Vallad GE, Ryder EJ, Subbarao KV (2006) Release of Iceberg lettuce germplasm with resistance to Verticillium Wilt. Agricultural Research Service, U.S. Dept. of Agriculture, USA

    Google Scholar 

  • Hayes RJ, Vallad GE, Qin Q-M, Grube RC, Subbarao KV (2007) Variation for resistance to Verticillium wilt in lettuce (Lactuca sativa L.). Plant Dis 91:439–445

    Article  Google Scholar 

  • Jansky S, Rouse DI, Kauth PJ (2004) Inheritance of resistance to Verticillium dahliae in diploid interspecific potato hybrids. Plant Dis 88:1075–1078

    Article  Google Scholar 

  • Kabir Z, Bhat RG, Subbarao KV (2004) Comparison of media for recovery of Verticillium dahliae from soil. Plant Dis 88:49–55

    Article  Google Scholar 

  • Kawchuk LM, Hachey J, Lynch DR, Kulcsar F, van Rooijen G, Waterer DR, Robertson A, Kokko E, Byers R, Howard RJ, Fischer R, Prüfer D (2001) Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci USA 98:6511–6515

    Article  PubMed  CAS  Google Scholar 

  • Ligoxigakis EK, Vakalounakis DJ, Thanassoulopoulos CC (2002) Weed hosts of Verticillium dahliae in Crete: susceptibility, symptomatology and significance. Phytoparasitica 30:511–518

    Article  Google Scholar 

  • Lynch DR, Kawchuk LM, Hachey J, Bains PS, Howard RJ (1997) Identification of a gene conferring high levels of resistance to Verticillium wilt in Solanum chacoense. Plant Dis 81:1011–1014

    Article  Google Scholar 

  • McHale LK, Truco MJ, Kozik A, Wroblewski T, Ochoa OE, Lahre KA, Knapp SJ, Michelmore RW (2009) The genomic architecture of disease resistance in lettuce. Theor Appl Genet 118:565–580

    Article  PubMed  CAS  Google Scholar 

  • Maruthachalam K, Atallah Z, Vallad GE, Klosterman SJ, Hayes RJ, Davis RM, Subbarao KV (2010) Molecular variation among isolates of Verticillium dahliae and PCR-based differentiation of races. Phytopathology 100:1222–1230

    Article  PubMed  CAS  Google Scholar 

  • Mert M, Kurt S, Gencer O, Akiscan Y, Boyaci K, Tok FM (2005) Inheritance of resistance to Verticillium wilt (Verticillium dahliae) in cotton (Gossypium hirsutum L.). Plant Breed 124:102–104

    Article  Google Scholar 

  • Pegg GF, Brady BL (2002) Verticillium wilts. CABI Publishing, New York

    Book  Google Scholar 

  • Putt E (1964) Breeding behavior of resistance to leaf mottle or Verticillium in sunflowers. Crop Sci 4:177–179

    Article  Google Scholar 

  • Ryder EJ (1971) Genetic studies in lettuce (Lactuca sativa L.). J Am Soc Hort Sci 96:826–828

    CAS  Google Scholar 

  • Ryder EJ, Johnson AS (1974) Mist depollination of lettuce flowers. HortScience 9:584

    Google Scholar 

  • Ryder EJ (1999) Lettuce, endive and chicory. Crop production science in horticulture series. CABI Publishing, New York

    Google Scholar 

  • Schaible L, Cannon OS, Waddoups V (1951) Inheritance of resistance to Verticillium wilt in a tomato cross. Phytopathology 41:986–990

    Google Scholar 

  • Simko I, Costanzo S, Haynes KG, Christ BJ, Jones RW (2004a) Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato (Solanum tuberosum) through a candidate gene approach. Theor Appl Genet 108:217–224

    Article  PubMed  CAS  Google Scholar 

  • Simko I, Haynes KG, Ewing EE, Costanzo S, Christ BJ, Jones RW (2004b) Mapping genes for resistance to Verticillium albo-atrum in tetraploid and diploid potato populations using haplotype association tests and genetic linkage analysis. Mol Genet Genomics 271:522–531

    Article  PubMed  CAS  Google Scholar 

  • Simko I, Haynes KG, Jones RW (2004c) Mining data from potato pedigrees: tracking the origin of susceptibility and resistance to Verticillium dahliae in North American cultivars through molecular marker analysis. Theor Appl Genet 108:225–230

    Article  PubMed  CAS  Google Scholar 

  • Simko I, Pechenick D, McHale LK, Truco MJ, Ochoa OE, Michelmore RW, Scheffler BE (2009) Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1. BMC Plant Biol 9:135

    Article  PubMed  Google Scholar 

  • Stam P, Van Ooijen JW (1995) Joinmap version 2.0: software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Subbarao KV, Hubbard JC, Greathead AS, Spencer GA (1997) Verticillium wilt. In: Davis RM, Subbarao KV, Raid RN, Kurtz EA (eds) Compendium of lettuce diseases. The American Phytopathological Society, St. Paul, pp 26–27

    Google Scholar 

  • Vallad GE, Bhat RG, Koike ST, Ryder EJ, Subbarao KV (2005) Weedborne reservoirs and seed transmission of Verticillium dahliae in lettuce. Plant Dis 89:317–324

    Article  Google Scholar 

  • Vallad GE, Qin Q-M, Grube RC, Hayes RJ, Subbarao KV (2006) Characterization of race-specific interaction among isolates of Verticillium dahliae pathogenic on lettuce. Phytopathology 96:1380–1387

    Article  PubMed  CAS  Google Scholar 

  • Vallad GE, Subbarao KV (2008) Colonization of resistant and susceptible lettuce cultivars by a green fluorescent protein-tagged isolate of Verticillium dahliae. Phytopathology 98:871–885

    Article  PubMed  CAS  Google Scholar 

  • van Bruggen AHC, Brown PR, Greathead A (1990) Distinction between infectious and noninfectious corky root of lettuce in relation to nitrogen fertilizer. J Am Soc Hort Sci 115:762–770

    Google Scholar 

Download references

Acknowledgments

This research was supported by the California Leafy Greens Research Program and the California Department of Food and Agriculture under the “Buy California Initiative” as well as an award to RWM from the National Research Initiative (NRI) Plant Genome Program of the USDA Cooperative State Research, Education and Extension Service (CSREES) Grant no. 04-35300-14601.

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Author notes

  1. Leah K. McHale

    Present address: Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, 43210, USA

Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Crop Improvement and Protection Unit, 1636 E. Alisal St, Salinas, CA, 93905, USA

    Ryan J. Hayes & Steve J. Klosterman

  2. The Genome Center and Department of Plant Sciences, University of California, Davis, CA, 95616, USA

    Leah K. McHale, Maria Jose Truco & Richard W. Michelmore

  3. Gulf Coast Research and Education Center, University of Florida, 14625 CR 672, Wimauma, FL, 33598, USA

    Gary E. Vallad

  4. Department of Plant Pathology, University of California, 1636 E. Alisal St, Salinas, CA, 93905, USA

    Karunakaran Maruthachalam & Krishna V. Subbarao

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  1. Ryan J. Hayes
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  2. Leah K. McHale
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  3. Gary E. Vallad
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  4. Maria Jose Truco
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  7. Karunakaran Maruthachalam
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  8. Krishna V. Subbarao
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Corresponding author

Correspondence to Ryan J. Hayes.

Additional information

Communicated by M. Havey.

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Hayes, R.J., McHale, L.K., Vallad, G.E. et al. The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theor Appl Genet 123, 509–517 (2011). https://doi.org/10.1007/s00122-011-1603-y

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