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Genetic analysis of resistance to bacterial leaf spot in the heirloom lettuce cultivar Reine des Glaces

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Abstract

Bacterial leaf spot (BLS) is a disease that affects lettuce (Lactuca sativa L.) worldwide. The disease is caused by the bacterium Xanthomonas campestris pathovar vitians (Xcv), which exclusively infects lettuce and is particularly devastating in warm humid climates. Reine des Glaces (RG), an old Batavia-type cultivar, exhibited an uninvestigated partial resistance to Xcv. Quantitative trait loci (QTLs) were analyzed using two recombinant inbred line (RIL) populations derived from RG × Eruption and RG × Delsay using three isolates collected in California and Canada, each representing a different race, and an isolate collected in France. A major QTL was identified in linkage group 2 in both populations; additional, minor QTLs were also detected in other linkage groups. The major QTL collocates with the Xanthomonas resistance 1 (Xar1) and Xanthomonas campestris vitians resistance (Xcvr) genes that had previously been identified using RIL populations derived from Salinas 88 × La Brillante and PI 358001-1 × Tall Guzmaine. RG provides another resistance resource for combating BLS using data provided by this study for marker-assisted selection.

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Acknowledgments

We thank Jose Orozco and Gabriel Ramos at the USDA in Salinas, California, and Adolfo Vargas, former junior specialist at the Genome Center, University of California, Davis, for technical assistance. Thanks also to Mathilde Hoerter at the INRA Research Unit, UR1052, Avignon (France) for the phenotyping of the RG x Delsay population. Thanks to Yunwen Wang, former University of Florida postdoc, who developed the mapping population PI 358001-1 × Tall Guzmaine. G.S. acknowledges the Hatch Project FLA-ERC-005599.

Funding

This research was funded by awards from the Specialty Crop Block Program in California award number SCB14044 and Florida award number 15SCBGPFL0038, the USDA NRI Specialty Crop Research Initiative award number 2015-51181-24283, and the California Leafy Greens Research Board. The phenotyping in France was supported by grants from Gautier Semences and Rijk Zwaan. The mentioning of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA.

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Authors and Affiliations

  1. The Genome Center, University of California, Davis, CA, USA

    Germán V. Sandoya, Maria Jose Truco & Richard W. Michelmore

  2. Horticultural Science Department, Everglades Research and Education Center, University of Florida, Belle Glade, Florida, USA

    Germán V. Sandoya

  3. GAFL, INRA, 84143, Montfavet, Avignon, France

    Brigitte Maisonneuve

  4. Department of Plant Pathology and Environmental Microbiology, Pennsylvania State University, University Park, PA, USA

    Carolee T. Bull

  5. USDA-ARS Crop Improvement and Protection Research Unit, Salinas, CA, USA

    Ivan Simko, Mark Trent & Ryan J. Hayes

  6. Mount Pleasant, Tinogona Foundation, Harare, Zimbabwe

    Mark Trent

  7. USDA-ARS Forage Seed and Cereal Research Unit, Corvallis, OR, USA

    Ryan J. Hayes

  8. Department of Plant Sciences, University of California, Davis, CA, USA

    Richard W. Michelmore

Authors
  1. Germán V. Sandoya
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  2. Brigitte Maisonneuve
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  3. Maria Jose Truco
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  4. Carolee T. Bull
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  5. Ivan Simko
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Correspondence to Germán V. Sandoya.

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Sandoya, G.V., Maisonneuve, B., Truco, M.J. et al. Genetic analysis of resistance to bacterial leaf spot in the heirloom lettuce cultivar Reine des Glaces. Mol Breeding 39, 160 (2019). https://doi.org/10.1007/s11032-019-1072-6

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