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Genetics of resistance in lettuce to races 1 and 2 of Verticillium dahliae from different host species

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Abstract

Race 1 resistance against Verticillium dahliae in lettuce was originally shown in the cultivar La Brillante to be conditioned by a single dominant gene (Verticillium resistance 1, Vr1). Multiple, morphologically diverse sources of germplasm have been identified as resistant to race 1. In this study, allelism tests indicated that resistance in these different lettuce cultivars is closely linked or allelic to the Vr1 gene. The Vr1 gene is defeated by race 2 isolates of V. dahliae. Only partial resistance to race 2 isolates is available in a few plant introductions (PIs). Greenhouse and field experiments conducted with these PIs demonstrated partial resistance to V. dahliae race 1 as well as race 2 isolates from lettuce. Cultivars resistant to race 1 and PIs with partial resistance to race 2 were challenged with several race 1 and 2 isolates originating from hosts other than lettuce. This indicated that cultivars resistant to race 1 and the breeding lines derived from them would also be resistant to race 1 isolates from other hosts; similarly, the partial resistance would be effective against race 1 and 2 isolates from hosts other than lettuce. Nevertheless, there were specific interactions that warrant further study. Although race 1 currently predominates in the major lettuce production area of the Salinas Valley, CA, breeding lettuce for resistance to V. dahliae should take both races into account.

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Acknowledgements

We extend special thanks to Crystal Marchebout and Rosa Marchebout, laboratory technicians from the Department of Plant Pathology at UC Davis and Jose Orozco and Dawn Avery of the USDA-ARS, Salinas, CA. This research was funded by USDA NIFA Grant # 59-5305-4-002 and USDA NIFA Specialty Crop Research Initiative (SCRI) Grant # 2015-51181-24283 and by The California Leafy Greens Research Board.

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Author notes

  1. German V. Sandoya

    Present address: Everglades Research Education Center, Horticultural Sciences Department – IFAS, University of Florida, Belle Glade, FL, 33430, USA

Authors and Affiliations

  1. The Genome Center and Department of Plant Sciences, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA

    German V. Sandoya & Richard W. Michelmore

  2. Everglades Research Education Center, Horticultural Sciences Department – IFAS, University of Florida, Belle Glade, FL, 33430, USA

    Richard W. Michelmore

  3. Department of Plant Pathology, University of California, Davis, 1636 E Alisal St., Salinas, CA, 93905, USA

    Suraj Gurung, Dylan P. Short & Krishna V. Subbarao

  4. Sakata Seed of America, Salinas, CA, 93907, USA

    Suraj Gurung

  5. Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, 26506, USA

    Dylan P. Short

  6. National Forage Seed Production Research Center, United States Department of Agriculture, USDA, 3450 SW Campus Way, Corvallis, OR, 97331, USA

    Ryan J. Hayes

Authors
  1. German V. Sandoya
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  2. Suraj Gurung
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  4. Krishna V. Subbarao
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  5. Richard W. Michelmore
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  6. Ryan J. Hayes
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Correspondence to Ryan J. Hayes.

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Sandoya, G.V., Gurung, S., Short, D.P. et al. Genetics of resistance in lettuce to races 1 and 2 of Verticillium dahliae from different host species. Euphytica 213, 20 (2017). https://doi.org/10.1007/s10681-016-1813-0

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