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Comparative mapping of the Oregon Wolfe Barley using doubled haploid lines derived from female and male gametes

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Abstract

The Oregon Wolfe Barley mapping population is a resource for genetics research and instruction. Prior reports are based on a population of doubled haploid (DH) lines developed by the Hordeum bulbosum (H.b.) method, which samples female gametes. We developed new DH lines from the same cross using anther culture (A.C.), which samples male gametes. Linkage maps were generated in each of the two subpopulations using the same 1,328 single nucleotide polymorphism markers. The linkage maps based on DH lines derived from the products of megasporogeneis and microsporogenesis revealed minor differences in terms of estimated recombination rates. There were no differences in locus ordering. There was greater segregation distortion in the A.C.-derived subpopulation than in the H.b.-derived subpopulation, but in the region showing the greatest distortion, the cause was more likely allelic variation at the ZEO1 plant height locus rather than to DH production method. The effects of segregation distortion and pleiotropy had greater impacts on estimates of quantitative trait locus effect than population size for reproductive fitness traits assayed under greenhouse conditions. The Oregon Wolfe Barley (OWB) population and data are community resources. Seed is available from three distribution centers located in North America, Europe, and Asia. Details on ordering seed sets, as well as complete genotype and phenotype data files, are available at http://wheat.pw.usda.gov/ggpages/maps/OWB/.

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Acknowledgments

L. Cistué was recipient of a Senior Research Fellowship from the Spanish Ministry of Science and Innovation during his time with the Oregon State University Barley Project and his research was financed by the Spanish Ministry of Science and Innovation thought the National Plan Projects AGL2005-07195-C02-01 and AGL2008-05541-C02-01. Alfonso Cuesta-Marcos was supported by a postdoctoral fellowship from the Spanish Ministerio de Ciencia e Innovación (MICINN). The BOPA SNPs were developed under the auspices of USDA-CSREES-NRI Grant No 2006- 55606-16722 “Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement”. This paper is dedicated to Dr. Bob Wolfe, whose vision and persistence led to the development of the Wolfe dominant and recessive marker stocks.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Oregon State University, Corvallis, OR, 97331, USA

    L. Cistué, A. Cuesta-Marcos, Y. Chutimanitsakun, A. Corey, T. Filichkina, N. Garcia-Mariño & P. M. Hayes

  2. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apartado 202, 50059, Zaragoza, Spain

    L. Cistué, B. Echávarri & N. Garcia-Mariño

  3. USDA-ARS Biosciences Research Lab, 1605 Albrecht Blvd, Fargo, ND, USA

    S. Chao

  4. Centre UdL-IRTA, Universitat de Lleida, Avda Alcalde Rovira Roure 91, 25198, Lleida, Spain

    I. Romagosa

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  1. L. Cistué
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  3. S. Chao
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  10. P. M. Hayes
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Correspondence to L. Cistué.

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Communicated by A. Graner.

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Cistué, L., Cuesta-Marcos, A., Chao, S. et al. Comparative mapping of the Oregon Wolfe Barley using doubled haploid lines derived from female and male gametes. Theor Appl Genet 122, 1399–1410 (2011). https://doi.org/10.1007/s00122-011-1540-9

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  • DOI: https://doi.org/10.1007/s00122-011-1540-9

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