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Identification and validation of QTLs for green plant percentage in barley (Hordeum vulgare L.) anther culture

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Abstract

In cereals, albinism is a major obstacle to produce doubled haploids (DH) for breeding programs. In order to identify QTLs for green plant percentage in barley anther culture, a specific population was developed. This population, consisting of 100 DH lines, was generated by crossing the model cultivar for anther culture “Igri” with an albino-producing DH line (DH46) selected from Igri × Dobla, in search of a maximum segregation for the trait and minimum for the other anther culture variables. A combination of bulked segregant analysis and AFLP methodology was used to identify markers linked to the trait. A linkage map was constructed using these AFLPs, together with RAPD, STS and SSR markers. This study identified a new QTL for green plant percentage on chromosome 3H and confirmed the previously reported one on chromosome 5H. Up to 65.2% of the phenotypic variance for this trait was explained by the additive effects of these two QTLs. Thirty elite cultivars of barley from different origin, row type, growth habit and end use, were selected to validate these QTLs. Since two of the markers linked to the QTLs were AFLPs, we successfully converted them into simple PCR-based SCAR markers. Only the SSR HVM60, on chromosome 3H, was significantly associated with the trait, explaining near 20% of the phenotypic variance. Among the allelic variants identified for this marker, HVM60-120bp was associated with the highest values of green plant percentage.

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Acknowledgements

We thank Dr. Salomé Prat for her help with the SCAR development. We are also grateful to C. Andrés, P. Devaux, B. Forster and S. Ullrich for providing the elite cultivar seeds. M. Muñoz-Amatriaín and X.-W. Chen were recipients of a fellowship from Ministry of Education and Science of Spain. The research was supported by Projects AGL2001-1631 and AGL2004-03396 from Plan Nacional de Recursos y Tecnologías Agroalimentarias of Spain.

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Authors and Affiliations

  1. Departamento de Genética y Producción Vegetal, Estación Experimental Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 202, 50080, Zaragoza, Spain

    M. Muñoz-Amatriaín, A. M. Castillo, L. Cistué & M. P. Vallés

  2. Department of Biochemistry and Molecular Biology, Nankai University, Tianjin, 300071, People’s Republic of China

    X. W. Chen

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  1. M. Muñoz-Amatriaín
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  2. A. M. Castillo
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  3. X. W. Chen
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  4. L. Cistué
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  5. M. P. Vallés
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Correspondence to M. P. Vallés.

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Muñoz-Amatriaín, M., Castillo, A.M., Chen, X.W. et al. Identification and validation of QTLs for green plant percentage in barley (Hordeum vulgare L.) anther culture. Mol Breeding 22, 119–129 (2008). https://doi.org/10.1007/s11032-008-9161-y

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  • DOI: https://doi.org/10.1007/s11032-008-9161-y

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