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Quantitative trait loci analysis for resistance to Cephalosporium stripe, a vascular wilt disease of wheat

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Abstract

Cephalosporium stripe, caused by Cephalosporium gramineum, can cause severe loss of wheat (Triticum aestivum L.) yield and grain quality and can be an important factor limiting adoption of conservation tillage practices. Selecting for resistance to Cephalosporium stripe is problematic; however, as optimum conditions for disease do not occur annually under natural conditions, inoculum levels can be spatially heterogeneous, and little is known about the inheritance of resistance. A population of 268 recombinant inbred lines (RILs) derived from a cross between two wheat cultivars was characterized using field screening and molecular markers to investigate the inheritance of resistance to Cephalosporium stripe. Whiteheads (sterile heads caused by pathogen infection) were measured on each RIL in three field environments under artificially inoculated conditions. A linkage map for this population was created based on 204 SSR and DArT markers. A total of 36 linkage groups were resolved, representing portions of all chromosomes except for chromosome 1D, which lacked a sufficient number of polymorphic markers. Quantitative trait locus (QTL) analysis identified seven regions associated with resistance to Cephalosporium stripe, with approximately equal additive effects. Four QTL derived from the more susceptible parent (Brundage) and three came from the more resistant parent (Coda), but the cumulative, additive effect of QTL from Coda was greater than that of Brundage. Additivity of QTL effects was confirmed through regression analysis and demonstrates the advantage of accumulating multiple QTL alleles to achieve high levels of resistance.

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Acknowledgments

We thank Karl Rhinhart and Erling Jacobsen for excellent management of the field plots. This research was supported by a USDA STEEP grant.

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  1. Martin C. Quincke

    Present address: INIA La Estanzuela, Ruta, 50 km 11, CC 39173, Colonia, Uruguay

Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331, USA

    Martin C. Quincke, C. James Peterson & Oscar Riera-Lizarazu

  2. Limagrain Cereals Seeds, 3515 Richards Lake Road, Fort Collins, CO, 80524, USA

    C. James Peterson

  3. ICRISAT, Patancheru, 502 324, Andhra Pradesh, India

    Oscar Riera-Lizarazu

  4. Department of Plant, Soil, and Entomological Sciences, University of Idaho, Moscow, ID, 83844-2339, USA

    Robert S. Zemetra

  5. Department of Crop and Soil Science, Washington State University, Pullman, WA, 99164-6420, USA

    Jennifer L. Hansen

  6. Department of Plant, Soil and Entomological Sciences, University of Idaho Aberdeen Research and Extension Center, Aberdeen, ID, 83210-1749, USA

    Jianli Chen

  7. Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331-2902, USA

    Christopher C. Mundt

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Communicated by I. Mackay.

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Quincke, M.C., Peterson, C.J., Zemetra, R.S. et al. Quantitative trait loci analysis for resistance to Cephalosporium stripe, a vascular wilt disease of wheat. Theor Appl Genet 122, 1339–1349 (2011). https://doi.org/10.1007/s00122-011-1535-6

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