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Developing stable progenies of ×Brassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture

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Abstract

Induced mutations were used to improve the low seed fertility of an intergeneric allopolyploid, ‘Baemoochae,’ ×Brassicoraphanus, synthesized following hybridization between Brassica rapa and Raphanus sativus. The mutagen N-methyl-N-nitroso-urethane (NMU) was added to microspore cultures. Four lines of nine in the Mi2 generation showed very high fertility under controlled pollination. The progeny lines (Mi3) confirmed this result under open pollination, and excellent uniformity was observed in plants grown in the field, as well as in their AFLP profile. On attaining high fertility and uniformity, one of the lines was released to farmers as a new leafy vegetable crop. The original nine lines shared very similar AFLP banding patterns, without any large differences between the high and low seed fertility lines. Thus, mutation induction accelerated genetic stabilization of a newly synthesized allopolyploid, ×Brassicoraphanus.

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Acknowledgments

This work was supported by the Institute of Planning and Evaluation for Technology, Ministry of Food, Agriculture, Forestry, and Fisheries of Korea (506017-05-SB010) and AgriFood New Material Research Center, Gyonggi Regional Research Center, Chung-Ang University. We thank Dr. Prakash, National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, for reviewing and commenting on this manuscript. We would also like to thank the personnel at BBI for their assistance in this work.

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Authors and Affiliations

  1. BioBreeding Institute, Business Incubation, Chung-Ang University, Ansung, Gyounggi, Korea

    Soo-Seong Lee, Sun-Ae Lee & Jungmin Yang

  2. Department of Applied Plant Science, Chung-Ang University, Ansung, Gyounggi, Korea

    Jongkee Kim

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  1. Soo-Seong Lee
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  2. Sun-Ae Lee
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Correspondence to Soo-Seong Lee.

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Communicated by H. Becker.

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Lee, SS., Lee, SA., Yang, J. et al. Developing stable progenies of ×Brassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture. Theor Appl Genet 122, 885–891 (2011). https://doi.org/10.1007/s00122-010-1494-3

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