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Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels

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Abstract

Arabidopsis accessions Col-0 and C24 and their reciprocal hybrids were employed as a model system to investigate the potential relationship between changes in DNA methylation, chromatin structure, endopolyploidization and gene expression in heterotic genotypes. Nucleolus size, endopolyploidization level and distribution of DNA and histone H3 methylation at the microscopic level does not differ between parents and their hybrids. Methylation sensitive amplified polymorphism revealed a largely constant pattern of DNA methylation (97% of signals analyzed) after intraspecific crosses. The parental expression profile of selected genes was maintained in hybrid offspring. No correlation was found between expression pattern and DNA methylation levels at restriction sites within 5′ regulatory regions. Thus, the results revealed only minor changes of chromatin properties and other nuclear features in response to intraspecific hybridization in Arabidopsis thaliana.

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Acknowledgments

We are grateful to O. Weiß and K. Kumke for technical assistance. We are grateful to A. Meister, R. Pickering, H. Witucka-Wall, A. Blacha, and I. Schubert, for helpful discussions. This work was supported by Deutsche Forschungsgemeinschaft (DFG) grant HO 1779/7-1/2 within framework program SPP 1149 “Heterosis in Plants.”

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  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    Ali Mohammad Banaei Moghaddam, Jörg Fuchs, Tobias Czauderna, Andreas Houben & Michael Florian Mette

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  1. Ali Mohammad Banaei Moghaddam
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  2. Jörg Fuchs
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  3. Tobias Czauderna
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  4. Andreas Houben
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Correspondence to Michael Florian Mette.

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Communicated by H. H. Geiger.

Contribution to the special issue "Heterosis in Plants".

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Banaei Moghaddam, A.M., Fuchs, J., Czauderna, T. et al. Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels. Theor Appl Genet 120, 215–226 (2010). https://doi.org/10.1007/s00122-009-1127-x

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  • DOI: https://doi.org/10.1007/s00122-009-1127-x

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