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Genes and traits associated with chromosome 2H and 5H regions controlling sensitivity of reproductive tissues to frost in barley

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Abstract

Frost at flowering can cause significant damage to cereal crops. QTL for low temperature tolerance in reproductive tissues (LTR tolerance) were previously described on barley 2HL and 5HL chromosome arms. With the aim of identifying potential LTR tolerance mechanisms, barley Amagi Nijo × WI2585 and Haruna Nijo × Galleon populations were examined for flowering time and spike morphology traits associated with the LTR tolerance loci. In spring-type progeny of both crosses, winter alleles at the Vrn-H1 vernalization response locus on 5H were linked in coupling with LTR tolerance and were unexpectedly associated with earlier flowering. In contrast, tolerance on 2HL was coupled with late flowering alleles at a locus we named Flt-2L. Both chromosome regions influenced chasmogamy/cleistogamy (open/closed florets), although tolerance was associated with cleistogamy at the 2HL locus and chasmogamy at the 5HL locus. LTR tolerance controlled by both loci was accompanied by shorter spikes, which were due to fewer florets per spike on 5HL, but shorter rachis internodes on 2HL. The Eps-2S locus also segregated in both crosses and influenced spike length and flowering time but not LTR tolerance. Thus, none of the traits was consistently correlated with LTR tolerance, suggesting that the tolerance may be due to some other visible trait or an intrinsic (biochemical) property. Winter alleles at the Vrn-H1 locus and short rachis internodes may be of potential use in barley breeding, as markers for selection of LTR tolerance at 5HL and 2HL loci, respectively.

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Acknowledgments

We gratefully acknowledge the Grains Research and Development Corporation, the Australian Research Council, the University of Adelaide and the South Australian government for funding. We thank Peter Langridge for seeds and the molecular marker data set of the Amagi Nijo × WI2585 F1-derived doubled-haploid population, Jason Eglinton and Stewart Coventry for access to FIS data and helpful discussions and Paul Gooding for technical assistance.

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Authors and Affiliations

  1. Australian Centre for Plant Functional Genomics (ACPFG), School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

    Andrew Chen, Jason Reinheimer, Ute Baumann, Margaret Pallotta, Geoffrey B. Fincher & Nicholas C. Collins

  2. Plant Breeding Unit, Australian Grain Technologies, Roseworthy Campus, Roseworthy, SA, 5371, Australia

    Jason Reinheimer

  3. Department of Plant Science, University of Manitoba, 66 Dafoe Road, 222 Agriculture Building, Winnipeg, MB, R3T 2N2, Canada

    Anita Brûlé-Babel

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  1. Andrew Chen
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  4. Ute Baumann
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Correspondence to Nicholas C. Collins.

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Communicated by J. Snape.

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Chen, A., Reinheimer, J., Brûlé-Babel, A. et al. Genes and traits associated with chromosome 2H and 5H regions controlling sensitivity of reproductive tissues to frost in barley. Theor Appl Genet 118, 1465–1476 (2009). https://doi.org/10.1007/s00122-009-0995-4

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  • DOI: https://doi.org/10.1007/s00122-009-0995-4

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