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Detection of a quantitative trait locus controlling carbon isotope discrimination and its contribution to stomatal conductance in japonica rice

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Abstract

Increasing leaf photosynthesis offers a possible way to improve yield potential in rice (Oryza sativa L.). Carbon isotope discrimination (Δ13C) has potential as an indirect selection criterion. In this study, we searched for quantitative trait loci (QTLs) controlling Δ13C, and assessed their association with leaf photosynthesis. Substitution mapping by using chromosome segment substitution lines (CSSLs), that carry segments from the indica cultivar Kasalath in the genetic background of the japonica cultivar Koshihikari, identified genomic regions affecting Δ13C on chromosomes (Chr.) 2, 3, 6, 7, and 12. One of the CSSLs, SL208, in which most regions on Chr. 3 were substituted with Kasalath segments, showed higher leaf stomatal conductance for CO2 (g s) and Δ13C than Koshihikari during the vegetative stage although leaf photosynthetic rate did not differ between them. These results suggest an association between Δ13C and g s. To test this association, we performed a QTL analysis for Δ13C at vegetative and heading stages in an F2 population derived from a cross between SL208 and Koshihikari. The results confirmed a QTL controlling Δ13C on the long arm of Chr. 3. By using a near-isogenic line specific to Hd6, we ruled out the possibility that variation in Δ13C was generated through the pleiotropic effect of heading date.

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Acknowledgments

We thank the staff of the technical support section of NIAS and NICS for field management. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, QTL1002).

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Authors and Affiliations

  1. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Toshiyuki Takai, Toshio Yamamoto & Masahiro Yano

  2. National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Akihiro Ohsumi, Yumiko San-oh, Ma. Rebecca C. Laza & Motohiko Kondo

Authors
  1. Toshiyuki Takai
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  2. Akihiro Ohsumi
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  3. Yumiko San-oh
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  4. Ma. Rebecca C. Laza
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  5. Motohiko Kondo
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  6. Toshio Yamamoto
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  7. Masahiro Yano
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Corresponding author

Correspondence to Masahiro Yano.

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Communicated by L. Xiong.

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Takai, T., Ohsumi, A., San-oh, Y. et al. Detection of a quantitative trait locus controlling carbon isotope discrimination and its contribution to stomatal conductance in japonica rice. Theor Appl Genet 118, 1401–1410 (2009). https://doi.org/10.1007/s00122-009-0990-9

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  • DOI: https://doi.org/10.1007/s00122-009-0990-9

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