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Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice

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Abstract

To understand the genetic basis of yield-related traits of rice, we developed 39 chromosome segment substitution lines (CSSLs) from a cross between an average-yielding japonica cultivar, Sasanishiki, as the recurrent parent and a high-yielding indica cultivar, Habataki, as the donor. Five morphological components of panicle architecture in the CSSLs were evaluated in 2 years, and 38 quantitative trait loci (QTLs) distributed on 11 chromosomes were detected. The additive effect of each QTL was relatively small, suggesting that none of the QTLs could explain much of the phenotypic difference in sink size between Sasanishiki and Habataki. We developed nearly isogenic lines for two major QTLs, qSBN1 (for secondary branch number on chromosome 1) and qPBN6 (for primary branch number on chromosome 6), and a line containing both. Phenotypic analysis of these lines revealed that qSBN1 and qPBN6 contributed independently to sink size and that the combined line produced more spikelets. This suggests that the cumulative effects of QTLs distributed throughout the genome form the major genetic basis of panicle architecture in rice.

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Acknowledgments

The seeds and genotype information of the CSSLs are available from the Rice Genome Resource Center (http://www.rgrc.dna.affrc.go.jp/index.html) of NIAS. This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Green Technology Project DM-1003 and QT-1005).

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Author notes

  1. Xiu Fang Ma

    Present address: North Japonica Hybrid Rice Research and Development Center, Liaoning Rice Research Institution, Shenyang, Liaoning, 110101, China

  2. Yoshinobu Takeuchi

    Present address: National Institute of Crop Science, Tsukuba, Ibaraki, 305-8518, Japan

  3. Shao Yang Lin

    Present address: Honda Research Institute Japan, Kisarazu, Chiba, 292-0818, Japan

Authors and Affiliations

  1. Institute of the Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki, 305-0854, Japan

    Tsuyu Ando, Takehiko Shimizu, Ayahiko Shomura, Yoshinobu Takeuchi & Shao Yang Lin

  2. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Toshio Yamamoto, Xiu Fang Ma & Masahiro Yano

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  1. Tsuyu Ando
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  2. Toshio Yamamoto
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  3. Takehiko Shimizu
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  4. Xiu Fang Ma
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  5. Ayahiko Shomura
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  6. Yoshinobu Takeuchi
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  8. Masahiro Yano
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Corresponding author

Correspondence to Masahiro Yano.

Additional information

Communicated by Q. Zhang.

T. Ando and T. Yamamoto contributed equally to this work.

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Ando, T., Yamamoto, T., Shimizu, T. et al. Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice. Theor Appl Genet 116, 881–890 (2008). https://doi.org/10.1007/s00122-008-0722-6

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  • DOI: https://doi.org/10.1007/s00122-008-0722-6

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