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Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK

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Abstract

Using a forward genetics approach, we isolated two independent low phytic acid (lpa) rice mutants, N15-186 and N15-375. Both mutants are caused by single gene, recessive non-lethal mutations, which result in approximately 75% (N15-186) and 43% (N15-375) reductions in seed phytic acid (inositol hexakisphosphate). High-performance liquid chromatography and GC–MS analysis of seed extracts from N15-186 indicated that, in addition to phytic acid, inositol monophosphate was significantly reduced whereas inorganic phosphorus and myo-inositol were greatly increased when compared with wild-type. The changes observed in N15-186 resemble those previously described for the maize lpa3 mutant. Analysis of N15-375 revealed changes similar to those observed in previously characterized rice lpa1 mutants (i.e. significant reduction in phytic acid and corresponding increase in inorganic phosphorus with little or no change in inositol phosphate intermediates or myo-inositol). Further genetic analysis of the N15-186 mutant indicated that the mutation, designated lpa N15-186, was located in a region on chromosome 3 between the microsatellite markers RM15875 and RM15907. The rice orthologue of maize lpa3, which encodes a myo-inositol kinase, is in this interval. Sequence analysis of the N15-186 allele of this orthologue (Os03g52760) revealed a single base pair change (C/G to T/A) in the first exon of the gene, which results in a nonsense mutation. Our results indicate that lpa N15-186 is a mutant allele of the rice myo-inositol kinase (OsMIK) gene. Identification and characterization of lpa mutants, such as N15-186, will facilitate studies on the regulation of phytic acid biosynthesis and accumulation and help address questions concerning the contribution of the inositol lipid-dependent and independent biosynthetic pathways to the production of seed phytic acid.

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Acknowledgments

The project was supported by the National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service, grant number 2005-35301-15708 (T.H.T). We gratefully acknowledge technical assistance from P. M. Colowit and T. Caravello. We are also thankful to J. Neil Rutger and Victor Raboy for critical reading of the manuscript and helpful suggestions for improvement.

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Authors and Affiliations

  1. USDA-ARS Crops Pathology and Genetics Research Unit, Department of Plant Sciences, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA

    S. I. Kim & T. H. Tai

  2. Department of Plant Sciences, University of California at Davis, One Shields Avenue, Davis, CA, 95616, USA

    C. B. Andaya & S. S. Goyal

  3. USDA-ARS Western Human Nutrition Research Center, Department of Nutrition, University of California at Davis, 430 West Health Sciences Drive, Davis, CA, 95616, USA

    J. W. Newman

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  1. S. I. Kim
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  2. C. B. Andaya
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  3. J. W. Newman
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  4. S. S. Goyal
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  5. T. H. Tai
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Correspondence to T. H. Tai.

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Communicated by M. Xu.

The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Kim, S.I., Andaya, C.B., Newman, J.W. et al. Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK. Theor Appl Genet 117, 1291–1301 (2008). https://doi.org/10.1007/s00122-008-0863-7

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  • DOI: https://doi.org/10.1007/s00122-008-0863-7

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