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Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241)

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Abstract

Phytic acid, myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the major storage compound of phosphorous (P) in plants, predominantly accumulating in seeds (up to 4–5% of dry weight) and pollen. In cereals, phytic acid is deposited in embryo and aleurone grain tissues as a mixed "phytate" salt of potassium and magnesium, although phytates contain other mineral cations such as iron and zinc. During germination, phytates are broken down by the action of phytases, releasing their P, minerals and myo-inositol which become available to the growing seedling. Phytic acid represents an anti-nutritional factor for animals, and isolation of maize low phytic acid (lpa) mutants provides a novel approach to study its biochemical pathway and to tackle the nutritional problems associated with it. Following chemical mutagenesis of pollen, we have isolated a viable recessive mutant named lpa 241 showing about 90% reduction of phytic acid and about a tenfold increase in seed-free phosphate content. Although germination rate was decreased by about 30% compared to wild-type, developement of mutant plants was apparentely unaffected. The results of the genetic, biochemical and molecular characterization experiments carried out by SSR mapping, MDD-HPLC and RT-PCR are consistent with a mutation affecting the MIPS1S gene, coding for the first enzyme of the phytic acid biosynthetic pathway.

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Acknowledgements

This work was supported by the Ministero dell'Universita e della Ricerca Scientifica e Tecnologica-Cofin 1998 (to G.G.). We wish to thank Mikael Blom Sorensen (Risoe National Laboratory, DK) for skilful assistance in MDD-HPLC experiments, and Dr. Anne Marie Lescure (Laboratoire de Biologie des Semences, INRA INA-PG, Versailles, F) for kindly providing phyS11 cDNA clones.

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Authors and Affiliations

  1. Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133, Milano, Italy

    R. Pilu, D. Panzeri, G. Gavazzi & G. Consonni

  2. Risoe National Laboratory, Plant Research Department, P.O. Box 49, 4000, Roskilde, Denmark

    S. K. Rasmussen

  3. Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Ferrata 1, 27100, Pavia, Italy

    E. Nielsen

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  1. R. Pilu
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  2. D. Panzeri
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  3. G. Gavazzi
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  4. S. K. Rasmussen
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  5. G. Consonni
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  6. E. Nielsen
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Correspondence to E. Nielsen.

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Communicated by F. Salamini

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Pilu, R., Panzeri, D., Gavazzi, G. et al. Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241). Theor Appl Genet 107, 980–987 (2003). https://doi.org/10.1007/s00122-003-1316-y

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  • DOI: https://doi.org/10.1007/s00122-003-1316-y

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