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Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.)

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Abstract

Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate), or its salt form, phytate, is commonly regarded as the major anti-nutritional component in cereal and legume grains. Breeding of low phytic acid (lpa) crops has recently been considered as a potential way to increase nutritional quality of crop products. In this study, eight independent lpa rice mutant lines from both indica and japonica subspecies were developed through physical and chemical mutagenesis. Among them, five are non-lethal while the other three are homozygous lethal. None of the lethal lines could produce homozygous lpa plants through seed germination and growth under field conditions, but two of them could be rescued through in vitro culture of mature embryos. The non-lethal lpa mutants had lower PA content ranging from 34 to 64% that of their corresponding parent and four of them had an unchanged total P level. All the lpa mutations were inherited in a single recessive gene model and at least four lpa mutations were identified mutually non-allelic, while the other two remain to be verified. One mutation was mapped on chromosome 2 between microsatellite locus RM3542 and RM482, falling in the same region as the previously mapped lpa1-1 locus did; another lpa mutation was mapped on chromosome 3, tightly linked to RM3199 with a genetic distance of 1.198 cM. The latter mutation was very likely to have happened to the LOC_Os03g52760, a homolog of the maize myo-inositol kinase (EC 2.7.1.64) gene. The present work greatly expands the number of loci that could influence the biosynthesis of PA in rice, making rice an excellent model system for research in this area.

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Acknowledgments

The authors are grateful to Prof. Cui HR for his advice on molecular mapping and to Dr. Zhu Y and Dr Zhang JJ of Zhejiang University for assistance in PA–P determination using HPIC. We are grateful to Profs. Gao MW and Altosaar I for their critical reading of and constructive suggestions to this paper. This work was mainly supported by the Sixth EU Framework Programme (NOFORISK, FP6-506387), with supplementary funding from China Natural Science Foundation (30571131) and Zhejiang Provincial Department of Science and Technology (2005C32004). This project is also a part of the IAEA regional technical cooperation project (RAS07014).

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Authors and Affiliations

  1. IAEA-Zhejiang University Collaborating Center, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China

    Qing-Long Liu, Xiu-Hong Xu, Xue-Liang Ren, Hao-Wei Fu, Dian-Xing Wu & Qing-Yao Shu

  2. Institute of Crop Science and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Qing-Long Liu

  3. Jiaxing Academy of Agricultural Sciences, Jiaxing, Zhejiang, 314016, China

    Hao-Wei Fu

  4. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria

    Qing-Yao Shu

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  1. Qing-Long Liu
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Correspondence to Qing-Yao Shu.

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Communicated by T. Sasaki.

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Liu, QL., Xu, XH., Ren, XL. et al. Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.). Theor Appl Genet 114, 803–814 (2007). https://doi.org/10.1007/s00122-006-0478-9

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  • DOI: https://doi.org/10.1007/s00122-006-0478-9

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