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Small-scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus y

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Abstract

The coat protein (CP) gene of the potato virus Y (PVY) strain N605 has been cloned into a plant binary expression vector and introduced into the potato variety Bintje. The transformed lines, Bt6, that contained two copies of the CP gene showed complete resistance to the homologous strain PVY-N605 and a good resistance to the related strain PVY-O803 in the greenhouse. The good resistance of Bt6 to primary and secondary infections by PVY was confirmed in two successive field tests where the virus was transmitted by its natural aphid vector.

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References

  1. Bevan MW, Mason SE, Goelet P: Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed byAgrobacterium. EMBO J 4: 1921–1926 (1985).

    Google Scholar 

  2. Bravo-Almonacid F, Mentaberry AN: Nucleotide sequence coding for the PVYO coat protein. Nucl Acids Res 17: 4401 (1989).

    Google Scholar 

  3. Chasseray E, Duesing J: Field trials of transgenic plants: an overview. AGRO Food Industry hi-tech (1992).

  4. Collet GF, Malnoë P, Farinelli L, Reust W: Pommes de terre transgéniques au champ. Rev Suisse Agric 25: 373–381 (1993).

    Google Scholar 

  5. Cuozzo M, O'Connell KM, Kaniewski W, Fang R-X, Chua N-H, Tumer NE: Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6: 549–557 (1988).

    Google Scholar 

  6. Dalmay T, Balàzs E: Nucleotide sequence of an altered virulence potato virus Y coat protein gene (PVYH strain). Nucl Acids Res 18: 6721 (1991).

    Google Scholar 

  7. DeBokx JA, Piron PGM: Relative efficiency of a number of aphid species in the transmission of potato virus Yn in the Netherlands. Neth J Plant Path 96: 237–246 (1990).

    Google Scholar 

  8. Farinelli L, Malnoë P: Coat protein gene-mediated resistance to potato virus Y (PVY) in tobacco: examination of the resistance mechanisms. Is the transgenic coat protein required for protection? Mol Plant-Microbe Interact 6: 284–293 (1993).

    Google Scholar 

  9. Farinelli L, Malnoë P, Collet GF: Heterologous encapsidation of potato virus Y strain O (PVYO) with the transgenic coat protein of PVY strain N (PVYN) inSolanum tuberosum cv. Bintje. Bio/Technology 10: 1020–1025 (1992).

    Google Scholar 

  10. Gugerli P: Potato viruses. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis, vol 11. Antigens and Antibodies 2, pp. 430–446. VCH Verlagsgesellschaft, Weinheim, FRG (1986).

    Google Scholar 

  11. Gugerli P, Fries P: Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J Gen Virol 64: 2471–2477 (1983).

    Google Scholar 

  12. Hay JM, Fellowes AP, Timmerman GM: Nucleotide sequence of the coat protein gene of a necrotic strain of potato virus Y from New Zealand. Arch Virol 107: 111–122 (1989).

    Google Scholar 

  13. Hemenway C, Fang R-X, Kaniewski WK, Chua N-H, Tumer NE: Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO J 7: 1273–1280 (1988).

    Google Scholar 

  14. Hollings M, Brunt AA: Potyvirus group. CMI/AAB Descriptions of Plant Viruses No. 245, 7 pp. (1981).

  15. Hoekema A, Huisman MJ, Molendijk L, van denElzen PJM, Cornelissen BJC: The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technology 7: 273–278 (1989).

    Google Scholar 

  16. Jongedijk E, deSchutter AAJM, Stolte T, van denElzen PJM, Cornelissen BJC: Increased resistance to potato virus X and preservation of cultivar properties in transgenic potato under field conditions. Bio/Technology 10: 422–429 (1992).

    Google Scholar 

  17. Kaniewski W, Lawson C, Sammons B, Haley L, Hart J, Delannay X, Tumer NE: Field resistance of transgenic Russet Burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8: 750–754 (1990).

    Google Scholar 

  18. Kawchuk LM, Martin RR, McPherson J: Resistance in transgenic potato expressing the potato leafroll virus coat protein gene. Mol Plant-Microbe Interact 3: 301–307 (1990).

    Google Scholar 

  19. Koncz C, Martini N, Mayerhofer R, Koncz-Kalman Z, Körber H, Redei GP, Schell J: High-frequency T-DNA-mediated gene tagging in plants. Proc Natl Acad Sci USA 86: 8467–8471 (1989).

    Google Scholar 

  20. Lawson C, Kaniewski W, Haley L, Rozman R, Newell C, Sanders P, Tumer NE: Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8: 127–134 (1990).

    Google Scholar 

  21. Lindbo JA, Dougherty WG: Pathogen-derived resistance to a potyvirus: immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. Mol Plant-Microbe Interact 5: 144–153 (1992).

    Google Scholar 

  22. Ling K, Namba S, Gonsalves C, Slightom JL, Gonsalves D: Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene. Bio/Technology 9: 752–758 (1991).

    Google Scholar 

  23. Loesch-Fries S, Merlo D, Zinnen T, Burhop L, Hill K, Krahn K, Jarvis N, Nelson S, Halk E: Expression of Alfalfa Mosaic Virus RNA 4 in transgenic plants confers virus resistance. EMBO J 6: 1845–1851 (1987).

    Google Scholar 

  24. Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).

    Google Scholar 

  25. Nemecek T: The role of aphid behaviour in the epidemology of potato virus Y: a simulation study. Thesis 10086, ETH, Zürich, Switzerland (1900).

  26. Peters D, Brooijmans E, Grohhuis PFM: Mobility as a factor in the efficiency with wich the aphids can spread nonpersistently transmitted viruses. Proc Exp Appl Entomol, N.E.V. Amsterdam, 1: 190–194 (1990).

    Google Scholar 

  27. Powell Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN: Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743 (1986).

    Google Scholar 

  28. Puurand U, Saarma M: Cloning and sequencing of the 3′-terminal region of potato virus YN (Russian isolate) RNA genome. Nucl Acids Res 18: 6694 (1991).

    Google Scholar 

  29. Quemada HD, Gonsalves D, Slightom JL: Expression of the coat protein gene from cucumber mosaic virus strain C in tobacco: protection against infections by CMV strains transmitted mechanically or by aphids. Phytopathology 81: 794–802 (1991).

    Google Scholar 

  30. Restrepo MA, Freed DD, Carrington JC: Nuclear transport of plant potyviral proteins. Plant Cell 2: 987–998 (1990).

    Google Scholar 

  31. Reust W, Le CL: La multiplication rapide des pommes de terre par le microbouturage. Revue Suisse Agric 17: 11–18 (1985).

    Google Scholar 

  32. Riechmann JL, Laín S, García JA: Highlights and prospects of potyvirus molecular biology. J Gen Virol 73: 1–16 (1992).

    Google Scholar 

  33. Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F: Nucleotide sequence of potato virus Y (N strain) genomic RNA. J Gen Virol 70: 935–947 (1989).

    Google Scholar 

  34. Stark DM, Beachy RN: Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262 (1989).

    Google Scholar 

  35. Thole V, Dalmay T, Burgyàn J, Balàzs E: Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123: 149–156 (1993).

    Google Scholar 

  36. Tumer NE, O'Connel M, Nelson RS, Sanders PR, Beachy RN, Fraley RT, Shah DM: Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO J 6: 1181–1188 (1987).

    Google Scholar 

  37. van derVlugt R, Allefs S, deHaan P, Goldbach R: Nucleotide sequence of the 3′-terminal region of potato virus Y mRNA. J Gen Virol 70: 229–233 (1989).

    Google Scholar 

  38. vanDun CMP, Overduin B, vanVloten-Doting L, Bol JF: Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164: 383–389 (1988).

    Google Scholar 

  39. Wefels E, Sommer H, Salamini F, Rohde W: Cloning of the potato virus Y genes encoding the capsid protein CP and the nuclear inclusion protein NIb. Arch Virol 107: 123–134 (1989).

    Google Scholar 

  40. Wilson TMA: Strategies to protect crop plants against viruses: Pathogen-derived resistance blossoms. Proc Natl Acad Sci USA 90: 3134–3141 (1993).

    Google Scholar 

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Malnoë, P., Farinelli, L., Collet, G.F. et al. Small-scale field tests with transgenic potato, cv. Bintje, to test resistance to primary and secondary infections with potato virus y. Plant Mol Biol 25, 963–975 (1994). https://doi.org/10.1007/BF00014670

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  • DOI: https://doi.org/10.1007/BF00014670

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