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Assessment of the importance of α-amylase inhibitor-2 in bruchid resistance of wild common bean

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Abstract

Both α-amylase inhibitor-2 (αAI-2) and arcelin have been implicated in resistance of wild common bean (Phaseolus vulgaris L.) to the Mexican bean weevil (Zabrotes subfasciatus Boheman). Near isogenic lines (NILs) for arcelin 1–5 were generated by backcrossing wild common bean accessions with a cultivated variety. Whereas seeds of a wild accession (G12953) containing both αAI-2 and arcelin 4 were completely resistant to Z. subfasciatus, those of the corresponding NIL were susceptible to infestation, suggesting that the principal determinant of resistance was lost during backcrossing. Three independent lines of transgenic azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] expressing αAI-2 accumulated high levels of this protein in seeds. The expression of αAI-2 in these lines conferred protection against the azuki bean weevil (Callosobruchus chinensis L.), likely through inhibition of larval digestive α-amylase. However, although the seed content of αAI-2 in these transgenic lines was similar to that in a wild accession of common bean (G12953), it did not confer a level of resistance to Z. subfasciatus similar to that of the wild accession. These results suggest that αAI-2 alone does not provide a high level of resistance to Z. subfasciatus. However, αAI-2 is an effective insecticidal protein with a spectrum of activity distinct from that of αAI-1, and it may prove beneficial in genetic engineering of insect resistance in legumes.

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Acknowledgments

This research was supported by the Project for Development of Innovative Transgenic Plants of the Ministry of Agriculture, Forestry, and Fisheries of Japan. We thank Koichi Fujii (University of Tsukuba) for providing colonies of Z. subfasciatus, Elizabeth E. Hood (ProdiGene, College Station, TX) for providing A. tumefaciens strain EHA105, and Yumi Nakamoto for technical assistance.

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Authors and Affiliations

  1. National Agricultural Research Center for Hokkaido Region, 1-Hitsujigaoka, Toyohira, Sapporo, Hokkaido, 062-8555, Japan

    Keito Nishizawa & Masao Ishimoto

  2. Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan

    Keito Nishizawa & Shigeru Utsumi

  3. Laboratory of Plant Production Control, Graduate School of Agriculture, Kyoto University, Takatsuki, Osaka, 569-0096, Japan

    Masayoshi Teraishi

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  1. Keito Nishizawa
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  2. Masayoshi Teraishi
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  3. Shigeru Utsumi
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  4. Masao Ishimoto
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Correspondence to Masao Ishimoto.

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Communicated by F. J. Muehlbauer.

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Nishizawa, K., Teraishi, M., Utsumi, S. et al. Assessment of the importance of α-amylase inhibitor-2 in bruchid resistance of wild common bean. Theor Appl Genet 114, 755–764 (2007). https://doi.org/10.1007/s00122-006-0476-y

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  • DOI: https://doi.org/10.1007/s00122-006-0476-y

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