Summary
SDS-PAGE of seed proteins from the seeds of a nondomesticated bean of Mexican origin (Phaseolus vulgaris L., PI 325690) revealed the presence of a novel 38 kd protein which appeared to be neither an altered phaseolin nor a lectin fraction. The protein was named arcelin, after Arcelia, the town in the state of Guerrero near which PI 325690 had been collected. The pure line, UW 325, was derived by self fertilization of the plant from a single arcelin-containing seed of PI 325690. Despite a low percentage seed phaseolin (14.6%), seed phenotype, seed germination, plant growth, pollen fertility, and percentage seed protein of UW 325 were normal. Analyses of F2 and F3 seeds from a single F1 plant of the cross ‘Sanilac’XPI 325690-3 revealed that arcelin expression was inherited as a single gene and that presence was dominant to absence of arcelin. The mean percentage phaseolin in the seeds of homozygous dominant Arc/Arc F3 families (14.0%) was significantly lower than that of the homozygous recessive arc/arc seeds (44.7%). The distribution of percentage phaseolin values for seeds within segregating families was bimodal and nonoverlapping. Without exception, seeds containing arcelin (Arc+phenotype) contained a lower percentage phaseolin than seeds lacking arcelin (Arc-phenotype). Although arcelin presence was associated with low percentage phaseolin, the Arc/Arc and Arc/arc genotypes were similar for seed weight and percentage total seed protein.
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Association of Official Agricultural Chemists (1960) Official methods of analysis, 9th edn. The Association, Washington DC, p 643
Blake T, Bliss FA (1983) Purification and characterization of a novel major bean seed protein. Agron Abstr, p 55
Brown JWS, Osborn TC, Bliss FA, Hall TC (1981) Genetic variation in the subunits of globulin-2 and albumin seed proteins of French bean. Theor Appl Genet 60:245–250
Brucher O (1968) Absence of phytohaemagglutinin in wild and cultivated beans from South America. Proc Tropical Region Am Soc Hortic Sci 12:68–85
Gentry HS (1969) Origin of the common bean, Phaseolus vulgaris. Econ Bot 23:55–69
Gepts P, Bliss FA (1984) Enhanced available methionine concentration associated with higher phaseolin levels in common bean seeds. Theor Appl Genet 69:47–53
Hartana A (1983) Genetic variability in seed protein levels associated with two phaseolin protein types in common bean (Phaseolus vulgaris L.). MSc Thesis, University of Wisconsin, Madison
Kelly JD, Bliss FA (1975) Heritability estimates of percentage seed protein and available methionine and correlations with yield in dry beans. Crop Sci 15:753–757
Laurell CB (1965) Antigen antibody crossed electrophoresis. Anal Biochem 10:358–361
Laurell CB (1967) Quantitative immunoelectrophoresis in antibody containing agarose gel. In: Peters H (ed) Proteins in biological fluids, vol 14. Elsevier, Amsterdam, pp 499–502
Ma Y, Bliss FA (1978) Seed proteins of common bean. Crop Sci 17:431–437
McLeester RC, Hall TC, Sun SM, Bliss FA (1973) Comparison of globulin proteins from Phaseolus vulgaris with those of Vicia faba. Phytochemistry 2:85–93
Mutschler MA (1979) The genetic control of globulin-1 seed protein and its relationship to total protein content and quality in dry bean (Phaseolus vulgaris L.); and male sterility in the dry bean (Phaseolus vulgaris L.). PhD Thesis, University of Wisconsin, Madison
Mutschler MA, Bliss FA (1981) Inheritance of bean seed globulin content and its relationship to protein content and quality. Crop Sci 21:289–294
Osborn TC, Bliss FA (1985) Effects of genetically removing lectin seed protein on horticultural and seed characteristics of common bean. J Am Soc Hortic Sci 110:484–488
Osborn TC, Brown JWS, Bliss FA (1985) Bean lectin. 5. Quantitative genetic variation in seed lectins of Phaseolus vulgaris and its relationship to qualitative lectin variation. Theor Appl Genet 70:22–31
Osborn TC, Mannen J-F, Brown JWS, Bliss FA (1984) Bean lectin. 4. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars. Theor Appl Genet 67:547–552
Romero-Andreas J (1984) Genetic variability in the seed phaseolin of nondomesticated bean (Phaseolus vulgaris var. ‘aborigineus’) and the inheritance and physiological effects of arcelin, a novel seed protein. PhD Thesis, University of Wisconsin, Madison
Sokal RR, Rohlf FJ (1981) Assumptions of analysis of variance. In: Biometry. Freeman, San Francisco, pp 418–419
Sullivan JG, Bliss FA (1983 a) Expression of enhanced seed protein content in inbred backcross lines of common bean. J Am Soc Hortic Sci 108:787–791
Sullivan JG, Bliss FA (1983b) Genetic control of quantitative variation in phaseolin seed protein of common bean. J Am Soc Hortic Sci 108:782–787
Sun SM, Hall TC (1975) Solubility characteristics of globulins from Phaseolus seeds in regard to their isolation and characterization. J Agric Food Chem 23:184–189
Sun SM, Mutschler MA, Bliss FA, Hall TC (1978) Protein synthesis and accumulation in bean cotyledons during growth. Plant Physiol 61:918–923
Weeke, B (1973) Rocket Immunoelectrophoresis. Scand J Immunol (Suppl 21) 2:37–46
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Communicated by D. von Wettstein
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Romero Andreas, J., Yandell, B.S. & Bliss, F.A. Bean arcelin. Theoret. Appl. Genetics 72, 123–128 (1986). https://doi.org/10.1007/BF00261467
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DOI: https://doi.org/10.1007/BF00261467