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Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight

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Abstract

Drought is the major constraint to chickpea (Cicer arietinum L.) productivity worldwide. Utilizing early-flowering genotypes and advancing sowing from spring to autumn have been suggested as strategies for drought avoidance. However, Ascochyta blight (causal agent: Didymella rabiei (Kov.) v. Arx.) is a major limitation for chickpea winter cultivation. Most efforts to introgress resistance to the pathogen into Kabuli germplasm resulted in relatively late flowering germplasm. With the aim to explore the feasibility of combining earliness and resistance, RILs derived from a cross between a Kabuli cultivar and a Desi accession were evaluated under field conditions and genotyped with SSR markers. Three quantitative trait loci (QTLs) with significant effects on resistance were identified: two linked loci located on LG4 in epistatic interaction and a third locus on LG8. Two QTLs were detected for time to flowering: one in LG1 and another on LG2. When resistance and time to flowering were analyzed together, the significance of the resistance estimates obtained for the LG8 locus increased and the locus effect on days to flowering, previously undetected, was significantly different from zero. The identification of a locus linked both to resistance and time to flowering may account for the correlation observed between these traits in this and other breeding attempts.

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Abbreviations

LOD:

Logarithm of the odds of linkage between two loci

PEV:

Proportion of explained phenotypic variance

QTL:

Quantitative trait locus (QTLs for loci)

RDR:

Relative response to Didymella rabiei

RIL:

Recombinant inbred line (RILs for lines)

SSR:

Simple sequence repeat

tRAUDPC:

Arcsin transformed area under the disease progress curve

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Acknowledgments

This research was supported in part by Research Grant Award US-3034-98R from The United States-Israel Binational Agricultural Research and Development Fund (BARD) awarded to S. Abbo, D. J. Bonfil, and H.-B. Zhang. The authors thank Mrs. H. Van-Oss for her skilful assistance in SSR genotyping, Mr. David Carlebakh and Agr. Israel Shai (Massuot-Yitzchak), Mr. Israel Mufradi and Mr. Saker El-Atrash (Gilat Station) and Dr. Pfaff for their assistance in the field experiments. The authors would also like to thank Prof. Cahaner and Prof. Korol for their professional advice.

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Author notes

  1. Judith Lichtenzveig

    Present address: Australian Centre for Necrotrophic Fungal Pathogens, Murdoch University, Murdoch, WA, 6150, Australia

Authors and Affiliations

  1. Institute of Plant Science and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel

    Judith Lichtenzveig & Shahal Abbo

  2. Plant Industry, CSIRO, Private Bag 5, Wembley, WA, 6913, Australia

    Judith Lichtenzveig

  3. Gilat Research Centre, Agricultural Research Organization, M.P. Negev 2, 85280, Israel

    David J. Bonfil

  4. Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, Texas A & M University, College Station, TX, 77843-2123, USA

    Hong-Bin Zhang

  5. Department of Plant Pathology, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel

    Dani Shtienberg

Authors
  1. Judith Lichtenzveig
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  2. David J. Bonfil
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  3. Hong-Bin Zhang
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  4. Dani Shtienberg
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  5. Shahal Abbo
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Corresponding author

Correspondence to Judith Lichtenzveig.

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Communicated by J.-L. Jannink.

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Lichtenzveig, J., Bonfil, D.J., Zhang, HB. et al. Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight. Theor Appl Genet 113, 1357–1369 (2006). https://doi.org/10.1007/s00122-006-0390-3

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