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Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm

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Abstract

Bulked segregant analysis combined with AFLPs was used to identify molecular markers linked to the Rca2 gene conferring resistance to Colletotrichum acutatum pathogenicity group 2 which causes anthracnose in the octoploid strawberry Fragaria × ananassa. DNA bulks originating from a cross between the resistant cultivar ‘Capitola’ and the susceptible cultivar ‘Pajaro’ were screened with 110 EcoRI/MseI AFLP combinations. Four AFLP markers were found linked in coupling phase to Rca2 with recombination percentages between 0% and 17.7%. Among the four markers linked to the resistance gene, two were converted into SCAR markers (STS-Rca2_417 and STS-Rca2_240) and screened in a large segregating population including 179 genotypes. The Rca2 resistance gene was estimated to be 0.6 cM from STS-Rca2_417 and 2.8 cM from STS-Rca2_240. The presence/absence of the two SCAR markers was further studied in 43 cultivars of F. × ananassa, including 14 susceptible, 28 resistant, and one intermediate genotype. Results showed that 81.4% and 62.8% of the resistant/susceptible genotypes were correctly predicted by using STS-Rca2_417 and STS-Rca2_240, respectively. The 14 susceptible genotypes showed no amplification for either SCARs. These developed SCARs constitute new tools for indirect selection criteria of anthracnose resistance genotypes in strawberry breeding programs.

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Acknowledgements

We would like to thank Dr. B. Smith for her critical review of the manuscript. The authors gratefully acknowledge funding from Région Aquitaine and the European Community (FEDER funds).

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  1. E. Lerceteau-Köhler

    Present address: Institut für Zoologie, Karl-Franzens Universität, Universitätsplatz 2, 8010, Graz, Austria

Authors and Affiliations

  1. Centre Interrégional de Recherche et d’Expérimentation de la Fraise (Ciref), Lanxade, 24130, Prigonrieux, France

    E. Lerceteau-Köhler

  2. INRA- Unité de Recherche sur les Espèces Fruitières et la Vigne, BP 81, 33883, Villenave d’Ornon Cedex, France

    G. Guérin & B. Denoyes-Rothan

Authors
  1. E. Lerceteau-Köhler
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  2. G. Guérin
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  3. B. Denoyes-Rothan
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Correspondence to B. Denoyes-Rothan.

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Communicated by H. Nybom

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Lerceteau-Köhler, E., Guérin, G. & Denoyes-Rothan, B. Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm. Theor Appl Genet 111, 862–870 (2005). https://doi.org/10.1007/s00122-005-0008-1

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  • DOI: https://doi.org/10.1007/s00122-005-0008-1

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