Abstract
Bulked segregant and AFLP analyses of two mapping populations (R17 × S6 and R17 × S1) were used to identify markers linked to Rpgm, the only known gene responsible for resistance to pine needle gall midge in Pinus thunbergii Parl. Rpgm was found to be bracketed by ACCC/CCTTT 190 on one side at a distance of 6.6 cM and ACGT/CCCGC 250 at 15.3 cM on the other side. The segregation of these markers was analyzed in two other families in order to determine their phase and transferability. One of the two additional resistant parents carried ACCC/CCTTT 190 in the homozygous state while the marker was in coupling (plus marker allele linked with an R allele) in a resistant parent, R17. The marker ACGT/CCCGC 250 was in a repulsion phase in R17 and was not detected in the other two resistant pine trees. Out of four AFLP markers identified, only ACGT/CCAAT 290 was transferable in all resistant trees tested, although its phase was opposite for different trees. These results indicate that in applying those markers to select resistant trees, the phase state of the markers in each resistant tree with respect to Rpgm needs to be considered.
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References
Baily NTJ (1961) Introduction to the mathematical theory of genetic linkage. Oxford University Press, London
Benet H, Guries RP, Boury S, Smalley EB (1995) Identification of RAPD markers linked to a black leaf spot resistance gene in Chinese elm. Theor Appl Genet 90:1068–1073
Cervera MT, Gusmao J, Steenackers M, Peleman J, Storme V, Vanden Broeck A, Van Montagu M, Boerjan W (1996) Identification of AFLP molecular markers for resistance against Melampsora larici-populina in Populus. Theor Appl Genet 93:733–737
Devey ME, Delfino-Mix A, Kinloch BB Jr, Neale DB (1995) Random amplified polymorphic DNA markers tightly linked to genes for resistance to white pine blister rust in sugar pine. Proc Natl Acad Sci USA 92:2066–2070
Ellis J, Dodds P, Pryor T (2000) Structure, function and evolution of plant disease/resistance genes. Curr Opin Plant Biol 3:278–284
Kawasaki S, Kamihara K, Motomura T, Kodama O (2000) Compact high density AFLP-enabled high efficiency genome scanning. In: 4th International Rice Genetics Symposium. Internation Rice Research Institute, p 73
Kondo T, Terada K, Hayashi E, Kuramoto N, Okamura M, Kawasaki H (2000) RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii). Theor Appl Genet 100:391–395
Kubisiak TL, Hebard FV, D. NC, Zhang J, Bernatzky R, Huang H, Anagnostakis SL, Doudrick RL (1997) Molecular mapping of resistance to blight in an interspecific cross in the genus Castanea. Phytopathology 87:751–759
Lander ES, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S, Newberg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lehner A, Campbell MA, Wheeler NC, Pöykkö T, Glössl J, Kreike J, Neale DB (1995) Identification of an RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula). Theor Appl Genet 91:1092–1094
Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. In: Technical report. Whitehead Institute, Cambridge, Mass.
Marques CM, Vasques-Kool J, Carocha VJ, Ferreira JG, O’Malley DM, Liu BH, Sederoff RR (1999) Genetic dissection of vegetative propagation traits in Eucalyptus tereticornis and E. globulus. Theor Appl Genet 99:939–946
Michelmore RW, Meyers BC (1998) Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process. Genome Res 8:1113–1130
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Murai H, Hashimoto Z, Sharma PN, Shimizu T, Murata K, Takumi S, Mori N, Kawasaki S, Nakamura C (2001) Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2. Theor Appl Genet 103:526–532
Newcombe G, Bradshaw HD Jr, Chastagner GA, Settler RF (1996) A major gene for resistance to Melampsora medusae f. sp. deltoidae in a hybrid poplar pedigree. Phytopathology 86:87–94
Remington DL, Whetten RW, Liu BH, O’Malley DM (1999) Construction of an AFLP genetic map with nearly complete genome coverage in Pinus taeda. Theor Appl Genet 98:1279–1292
Sone K (1986) Ecological studies on the pine needle gall midge, Thecodiplosis japonensis Uchida et Inouye (Diptera: Cecidomyiidae), I. Life history. Bull For For Prod Res Inst 341:1–25
Terada K (1992) Developing strains resistance to the pine needle gall midge (Thecodiplosis japonensis Uchida et Inouye) in Pinus thunbergii Parl. Bull For Tree Breed Inst 10:1–32
Travis SE, Ritland K, Whitham TG, Keim P (1998) A genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms. Theor Appl Genet 97:871–880
Tsuchiya T (1978) Selection of individuals resistant to pine needle gall midge (Thecodiplosis japonensis Uchida et Inouye). For Breed 108:16–17
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wilcox PL, Amerson HV, Kuhlman EG, Liu BH, O’Malley DM, Sederoff RR (1996) Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping. Proc Natl Acad Sci USA 93:3859–3864
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Communicated by D.B. Neale
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Hayashi, E., Kondo, T., Terada, K. et al. Identification of AFLP markers linked to a resistance gene against pine needle gall midge in Japanese black pine. Theor Appl Genet 108, 1177–1181 (2004). https://doi.org/10.1007/s00122-003-1537-0
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DOI: https://doi.org/10.1007/s00122-003-1537-0