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Chemopreventive effect of saponin isolated from Gymnema sylevestre on prostate cancer through in silico and in vivo analysis

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Abstract

Prostate cancer is one of the frequently diagnosed cancers in men. In prostate cancer, initiation to the appearance of symptoms is a very long period. Due to its high latency period it paved the way for the chemoprevention. Chemopreventive drugs quickly and effectively eliminate pre-malignant cells by inducing apoptosis. In this context, the present study utilized an integrated approach of in silico and in vivo analysis to find an effective chemopreventive drug, which induces apoptosis. Three phytocompounds from Gymnema sylvestre were screened against anti-apoptoic proteins (Bcl-2 and Bcl-xl) through in silico analysis and the best compound was identified as dihydroxy gymnemic triacetate. Its chemopreventive activity was analyzed in the N-methyl-N-nitrosourea and Testosterone-induced Sprague-Dawley rats. The result indicates that anti-apoptotic proteins (Bcl-2, Bcl-xl) level were significantly increased in cancer-induced animals compared to control whereas treatment with dihydroxy gymnemic triacetate supplementation significantly maintained the protein expression in par with control rats. This study suggests that dihydroxy gymnemic triacetate may act as a potential chemopreventive agent in targeting the prostate cancer. Thus, these findings may open novel prospective in cancer chemoprevention.

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Acknowledgements

The financial support extended by BIF Centre, DBT (Department of Biotechnology), Government of India and University Grants Commission. (Project No. 41-104/2012(SR)) are greatly acknowledged.

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Authors and Affiliations

  1. Bioinformatics Centre (BIF), PG & Research Department of Biotechnology & Bioinformatics, Holy Cross College (Autonomous), Tiruchirapalli, 620002, Tamil Nadu, India

    Rajamanickam Pon Nivedha & Manikkam Rajalakshmi

  2. Computer Aided Drug Design and Molecular Modelling Lab, Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India

    Venkatesan Suryanarayanan & Sanjeev Kumar Singh

  3. Department of Chemical Engineering, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul, Korea

    Chandrabose Selvaraj

Authors
  1. Rajamanickam Pon Nivedha
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  2. Venkatesan Suryanarayanan
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  3. Chandrabose Selvaraj
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  4. Sanjeev Kumar Singh
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  5. Manikkam Rajalakshmi
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Correspondence to Manikkam Rajalakshmi.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Pon Nivedha, R., Suryanarayanan, V., Selvaraj, C. et al. Chemopreventive effect of saponin isolated from Gymnema sylevestre on prostate cancer through in silico and in vivo analysis. Med Chem Res 26, 1915–1925 (2017). https://doi.org/10.1007/s00044-017-1900-3

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  • DOI: https://doi.org/10.1007/s00044-017-1900-3

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