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Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis

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Abstract

Objective

To investigate the effect of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis (SAP).

Methods

Rats were randomly divided into sham-operated, model control, and dexamethasone-treated groups. At 3, 6, and 12 h after operation, the expression levels of P-selectin protein in one-third of the rats in each group were observed.

Results

In the treated group, the expression levels of P-selectin protein in the pancreas head (at 6 h), lung (at 12 h), liver (at 3 h), and spleen (at 6 and 12 h) were significantly lower than those in the model control group (P < 0.05). Additionally, the products of the staining intensity and positive rate of P-selectin protein in liver (at 3 h), lung (at 6 and 12 h), and spleen (at 12 h) in the treated group were significantly lower than those in the model control group (P < 0.05).

Conclusions

Dexamethasone can inhibit P-selectin protein expression in multiple organs of SAP rats.

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Acknowledgments

This work was supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang province (No. 2003C130; No. 2004C142), Foundation Project for Medical Science and Technology of Zhejiang province (No. 2003B134), Grave Foundation Project for Technological and Development of Hangzhou (No. 2003123B19), Intensive Foundation Project for Technology of Hangzhou (No. 2004Z006), Foundation Project for Medical Science and Technology of Hangzhou (No. 2003A004), and Foundation Project for Technology of Hangzhou (No. 2005224). We state that this paper is original, that we have no financial interest in the company or its competitors, and that all authors meet the criteria for authorship. We abided by the ethics rules in this animal experiment study. Ethics committee approval of our hospital was secured, no rats were abused, and mercy killing was conducted when the observation time for this study was over.

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Authors and Affiliations

  1. Department of General Surgery, Hangzhou First People’s Hospital, 310006, Hangzhou, Zhejiang, China

    Zhang Xiping, Zhu Wei, Ye Jing, Jia Penghui, Yuan Wenqin, Zhou Ninnin & He Jiao

  2. Laboratory Animal Research Center, Zhejiang University, 310058, Hangzhou, Zhejiang, China

    Fu Jun

  3. Zhejiang University of Traditional Chinese Medicine, 310053, Hangzhou, Zhejiang, China

    Zhang Jie, Yu Bingyan & Ma Jing

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  1. Zhang Xiping
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  2. Fu Jun
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Correspondence to Zhang Xiping.

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Responsible Editor: A. Bauhofer.

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Xiping, Z., Jun, F., Jie, Z. et al. Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis. Inflamm. Res. 59, 31–39 (2010). https://doi.org/10.1007/s00011-009-0067-x

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  • DOI: https://doi.org/10.1007/s00011-009-0067-x

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