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Evaluation of the inflammatory response in sera from acute ischemic stroke patients by measurement of IL-2 and IL-10

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Abstract

Objectives

We compared the concentrations of the proinflammatory cytokine interleukin-2 (IL-2) with the anti-inflammatory cytokine interleukin-10 (IL-10) in serial serum samples from improved and expired acute ischemic stroke (AIS) patients to determine their prognostic usefulness.

Materials and methods

Blood from AIS patients (n = 17) admitted within 24 h of the onset of symptoms were collected at admission and 24, 48, 72, and 144 h after admission. Pro- and anti-inflammatory cytokines were measured by enzyme-linked immunosorbant assay.

Results

IL-2 levels were elevated in serum samples from AIS patients collected at 0 (0.97, P < 0.01) and 24 h (1.011, P < 0.01). IL-2 concentrations gradually decreased at subsequent time-points (48, 72, and 144 h samples, 0.324, P < 0.05) in patients who improved (n = 13), but not in those who expired (n = 4). Similarly, a decrease in IL-10 levels was observed in serum samples from AIS patients who improved at 24 h (0.180, P < 0.05), followed by significant increases at 72 h (0.97, P < 0.01 vs. control) and 144 h (1.38, P < 0.01).

Conclusion

The levels of IL-2 and IL-10 correlate well with outcome in AIS patients and may be useful in prognosis.

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Acknowledgment

We thank Mr. Prashant Deoras for assistance in statistical analyses.

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Authors and Affiliations

  1. Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India

    Amit R. Nayak, Rajpal S. Kashyap, Dinesh Kabra, Girdhar M. Taori & Hatim F. Daginawala

  2. Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India

    Hemant J. Purohit

Authors
  1. Amit R. Nayak
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  2. Rajpal S. Kashyap
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  3. Hemant J. Purohit
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  4. Dinesh Kabra
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  5. Girdhar M. Taori
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  6. Hatim F. Daginawala
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Correspondence to Hatim F. Daginawala.

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Responsible Editor: A. Bauhofer.

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Nayak, A.R., Kashyap, R.S., Purohit, H.J. et al. Evaluation of the inflammatory response in sera from acute ischemic stroke patients by measurement of IL-2 and IL-10. Inflamm. Res. 58, 687–691 (2009). https://doi.org/10.1007/s00011-009-0036-4

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  • DOI: https://doi.org/10.1007/s00011-009-0036-4

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