Abstract
Haynaldia villosa is a wild grass of the tribe Triticeae, other members of which include the cultivated cereals barley, rye, and wheat. We have made an electrophoretic and chemical characterization of the major seed storage proteins (prolamins) of H. villosa and determined the chromosomal locations of the structural genes for some components using the available wheat/H. villosa chromosome addition lines. As in wheat, barley, and rye, groups of high molecular weight (polymeric), sulfur-poor (monomeric), and sulfur-rich (monomeric γ-type and polymeric) prolamins can be recognized. Most of the components are encoded by genes on chromosome 1 Ha, which is homologous with the chromosomes controlling many of the prolamins in wheat and rye and all of those in barley. In addition, H. villosa also contains α-type sulfur-rich prolamins, previously detected only in wheat and its close relatives. These may be encoded by genes on chromosome 6Ha, which is homologous with the group 6 chromosomes that control the α-type gliadins of wheat. Despite the proposed close relationship between Haynaldia and ryes, no evidence was found for the presence of proteins closely related to the M r 75,000 γ-secalins which are characteristic of wild and cultivated species of Secale.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Autran, J.-C., Lew, E. J.-L., Nimmo, C. C., and Kasarda, D. D. (1979). N-Terminal amino acid sequencing of prolamins from wheat and related species, Nature (London) 282527.
Bartels, D., Altosaar, I., Harberd, N. P., Barker, R. F., and Thompson, R. D. (1986). Molecular analysis of γ-gliadin gene families at the complex Gli-1 locus of bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 72845.
Baum, B. R. (1977). Taxonomy of the tribe Triticeae (Poaceae) using various numerical techniques I. Can. J. Bot. 551712.
Baum, B. R. (1978a). Taxonomy of the tribe Triticeae (Poaceae) using various numerical techniques. II. Can. J. Bot. 5627.
Baum, B. R. (1978b). Taxonomy of the tribe Triticeae (Poaceae) using various numerical techniques. III. Can. J. Bot. 56374.
Baum, B. R. (1978c). Genetic relationships in Triticeae based on computations of Jardine & Sibson Bk clusters. Can. J. Bot. 562948.
Bietz, J. A., and Wall, J. S. (1980). Identity of high molecular weight gliadin and ethanol-soluble glutenin subunits of wheat: Relation to gluten structure. Cereal Chem. 57415.
Bietz, J. A., Huebner, F. R., Sandersen, J. E., and Wall, J. S. (1977). Wheat gliadin homology revealed through N-terminal amino acid sequence analysis. Cereal Chem. 541070.
Brandt, A., Montembault, A., Cameron-Mills, V., and Rasmussen, S. K. (1985). Primary structure of a B1 hordein gene from barley. Carlsberg Res. Commun. 50333.
Bunce, N., White, R. P., and Shewry, P. R. (1985). Variation in estimates of molecular weights of cereal prolamins by SDS-PAGE. J. Cereal Sci. 3131.
Della Gatta, C., Tanzarella, O. A., Resta, P., and Blanco, A. (1983). Protein content in a population of Haynaldia villosa and electrophoretic pattern of the amphiploid Triticum durum × Haynaldia villosa. In Porceddu, E. (ed.), Breeding Methodologies in Durum Wheat & Triticale Institute of Agricultural Biology, University of Tuscia, Italy, pp. 39–43.
Driscoll, C. J. (1983). Third compendium of wheat-alien chromosome lines. Suppl. Proc. 6th Int. Wheat Genet. Symp. Kyoto, Japan, 1983.
Dvorak, J., Kasarda, D. D., Dietler, M. D., Lew, E. J.-L., Anderson, O. W., Litts, J. C., and Shewry, P. R. (1986). Chromosomal location of seed storage protein genes in the genome of Elytrigia elongata. Can. J. Genet. Cytol. 28818.
Field, J. M., Shewry, P. R., and Miflin, B. J. (1983). Aggregation states of alcohol-soluble storage proteins of barley, rye, wheat and maize. J. Sci. Food Agr. 34362.
Forde, B., Heyworth, A., Pywell, J., and Kreis, M. (1985). Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat & maize. Nucl. Acids. Res. 137327.
Grenier, M., and Godron, M. (1856). Flore de France, Vol. 3 J. B. Baillière, Paris.
Hirs, C. H. W. (1967). Performic oxidation. Methods Enzymol. 11197.
Kasarda, D. D., Autran, J.-C., Lew, E. J.-L., Nimmo, C. C., and Shewry, P. R. (1983). N-terminal amino acid sequences of ω-gliadins and ω-secalins: Implications for the evolution of prolamin genes. Biochim. Biophys. Acta 747138.
Kasarda, D. D., Lafiandra, D., Morris, R., and Shewry, P. R. (1984a). Genetic relationships of wheat gliadin proteins. Kulturpflanze 32S33.
Kasarda, D. D., Okita, T. W., Bernardin, J. E., Baecker, P. A., Nimmo, C. C., Lew, E. J.-L., Dietler, M. D., and Greene, F. C. (1984b). Nucleic acid (cDNA) and amino acid sequences of α-type gliadins from wheat (Triticum aestivum). Proc. Natl. Acad. Sci. USA 814712.
Kreis, M., Shewry, P. R., Forde, B. G., Forde, J., and Miflin, B. J. (1985). Structure and evolution of seed storage proteins and their genes, with particular reference to those of wheat, barley and rye. In Miflin, B. J. (ed.), Oxford Surveys of Plant Cell and Molecular Biology, Vol. 2 Oxford, London, pp. 253–317.
Konarev, V. G. (1983). Plant Proteins as Genetic Markers Kolos, Moscow.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of heads of the bacteriophage T4. Nature (London) 227681.
Laursen, R. A. (1971). Solid phase Edman degradation. An automatic peptide sequence. Eur. J. Biochem. 2089.
Payne, P. I., Law, C. N., and Mudd, E. E. (1980). Control by homologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet. 58113.
Payne, P. I., Holt, L. M., Jackson, E. A., and Law, C. N. (1984). Wheat storage proteins: Their genetics and their potential for manipulation by plant breeding. Phil. Trans. R. Soc. Lond. B 304273.
Payne, P. I., Roberts, M. S., and Holt, L. M. (1986). Location of genes controlling the D group of LMW glutenin subunits on chromosome 1D of bread wheat. Genet. Res. Cambr. 47175.
Rahman, S., Shewry, P. R., and Miflin, B. J. (1982). Differential protein accumulation during barley grain development. J. Exp. Bot. 33717.
Scheets, K., Rafalski, J. A., Hedgcoth, C., and Soll, D. G. (1985). Heptapeptide repeat structure of a wheat γ-gliadin. Plant Sci. Lett. 37221.
Shewry, P. R., and Miflin, B. J. (1985). Seed storage proteins of economically important cereals. In Pomeranz, Y. (ed.), Advances in Cereal Science & Technology, Vol. VII AACC, St. Paul, Minn., pp. 1–83.
Shewry, P. R., Lew, E. J.-L., and Kasarda, D. D. (1981). Structural homology of storage proteins coded by the Hor 1 locus of barley (Hordeum vulgare L.). Planta 153246.
Shewry, P. R., Field, J. M., Lew, E. J.-L., and Kasarda, D. D. (1982). The purification and characterization of two groups of storage proteins (secalins) from rye (Secale cereale L.). J. Exp. Bot. 133261.
Shewry, P. R., Miflin, B. J., Lew, E. J.-L., and Kasarda, D. D. (1983). The preparation and characterization of an aggregated gliadin fraction from wheat. J. Exp. Bot. 341403.
Shewry, P. R., Bradberry, D., Franklin, J., and White, R. D. (1984a). The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theor. Appl. Genet. 6963.
Shewry, P. R., Miflin, B. J., and Kasarda, D. D. (1984b). The structural and evolutionary relationships of the prolamin storage proteins of barley, rye and wheat. Phil. Trans. R. Soc. Lond. B 34297.
Shewry, P. R., Kreis, M., Parmar, S., Lew, E. J.-L., and Kasarda, D. D. (1985). Identification of γ-type hordeins in barley. FEBS Lett. 19061.
Shewry, P. R., Parmar, S., Fulrath, N., Kasarda, D. D., and Miller, T. E. (1986). Chromosomal locations of the structural genes for secalins in wild perennial rye (Secale montanum Guss.) and cultivated rye (S. cereale L.) determined by two-dimensional electrophoresis. Can. J. Genet. Cytol. 2876.
Sugiyama, T., Rafalski, A., and Soll, D. (1986). The nucleotide sequence of a wheat γ-gliadin genomic clone. Plant Sci. 44205.
Wachter, E., Machleidt, W., Hofner, H., and Otto, J. (1973). Aminopropyl glass and its p-phenylene diisothiocyanate derivative, a new support in solid-phase Edman degradation of peptides and proteins. FEBS Lett. 3597.
Wrigley, C. W., Autran, J.-C., and Bushuk, W. (1982). Identification of cereal varieties by gel electrophoresis of the grain proteins. In Pomeranz, Y. (ed.), Advances in Cereal Science & Technology, Vol. 5 AACC, St. Paul, Minn., pp. 211–259.
Zimmerman, C. L., Appella, E., and Pisano, J. J. (1977). Rapid analysis of amino acid phenylthiohydantoins by high performance liquid chromatography. Anal. Biochem. 77569.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shewry, P.R., Parmar, S. & Pappin, D.J.C. Characterization and genetic control of the prolamins of Haynaldia villosa: Relationship to cultivated species of the Triticeae (rye, wheat, and barley). Biochem Genet 25, 309–325 (1987). https://doi.org/10.1007/BF00499323
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00499323