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Streptomyces seymenliensis sp. nov., isolated from soil

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Abstract

A novel actinomycete, designated strain B1041T, isolated from soil collected from the Tuz (Salt) Lake in the central Anatolia region, in Turkey, was characterised using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the members of the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree. Strain B1041T was found to be most closely related to Streptomyces plumbiresistens CCNWHX 13-160T (98.46 % sequence similarity), Streptomyces pseudovenezuelae NBRC 12904T (97.81 %), Streptomyces novaecaesareae NBRC 13368T (97.68 %), Streptomyces graminifolii JL-22T (97.60 %), Streptomyces phaeoluteigriseus NRRL ISP-5182T (97.58 %), Streptomyces ciscaucasicus NBRC 12872T (97.53 %) and Streptomyces pratensis ch24T (97.52 %). Sequence similarities with other strains of the genus Streptomyces were lower than 97.5 %. The cell wall of the novel strain was found to contain ll-diaminopimelic acid and the whole cell sugars were identified as galactose, glucose and ribose. The major cellular fatty acids were identified as anteiso C15:0 , iso C16:0 and anteiso C17:0. The predominant menaquinones found were found to be MK-9(H8) and MK-9(H6). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a phosphoglycolipid and two unknown phospholipids. The genomic DNA G+C content was determined to be 70.4 mol%. On the basis of the data from this polyphasic taxonomic study, strain B1041T can be considered to represent a novel species within the genus Streptomyces for which the name Streptomyces seymenliensis sp. nov. is proposed (type strain B1041T =KCTC 29245T = DSM 42117T).

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Acknowledgments

This research was supported by Ondokuz Mayis University (OMU), project no. PYO. FEN. 1901.11.011.

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Authors and Affiliations

  1. Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey

    Demet Tatar & Nevzat Sahin

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  1. Demet Tatar
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  2. Nevzat Sahin
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Correspondence to Nevzat Sahin.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of Streptomyces seymenliensis B1041T (=KCTC 29245T = DSM 42117T) is KC560729.

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Tatar, D., Sahin, N. Streptomyces seymenliensis sp. nov., isolated from soil. Antonie van Leeuwenhoek 107, 411–418 (2015). https://doi.org/10.1007/s10482-014-0339-5

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