Skip to main content
SpringerLink
Log in

Streptomonospora algeriensis sp. nov., a halophilic actinomycete isolated from soil in Algeria

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A halophilic actinomycete strain, designated H27T, was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10–15 % (w/v) and the optimum growth temperature and pH were found to be 28–37 °C and 6–7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27T were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27T are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27T is most closely related to Streptomonospora alba DSM 44588T (98.8 %) and Streptomonospora flavalba DSM 45155T (98.7 %) whereas the DNA–DNA relatedness values between strain H27T and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27T should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27T (=DSM 45604T =CCUG 63369T =MTCC 11563T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. J Appl Microbiol 12:421–423

    CAS  Google Scholar 

  • Cai M, Zhi XY, Tang SK, Zhang YQ, Xu LH, Li WJ (2008) Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. Int J Syst Evol Microbiol 58:1556–1560

    Article  CAS  PubMed  Google Scholar 

  • Cai M, Tang SK, Chen YG, Li Y, Zhang YQ, Li WJ (2009) Streptomonospora amylolytica sp. nov. and Streptomonospora flavalba sp. nov., two novel halophilic actinomycetes isolated from a salt lake. Int J Syst Evol Microbiol 59:2471–2475

    Article  CAS  PubMed  Google Scholar 

  • Chun J, Bae KS, Moon EY, Jung SO, Lee HK, Kim SJ (2000) Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913

    CAS  PubMed  Google Scholar 

  • Coenye T, Falsen E, Vancanneyt M, Hoste B, Govan JR, Kersters K, Vandamme P (1999) Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413

    Article  PubMed  Google Scholar 

  • Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    CAS  PubMed  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1977) On the problem of discovering the most parsimonious tree. Am Nat 111:223–257

    Article  Google Scholar 

  • Goodfellow M (1971) Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–90

    Article  CAS  PubMed  Google Scholar 

  • Gordon RE, Barnett DA (1977) Resistance to rifampicin and lysozyme of strains of some species of Mycobacterium and Nocardia as a taxonomic tool. Int J Syst Bacteriol 27:176–178

    Article  CAS  Google Scholar 

  • Gordon RE, Barnett DA, Handerhan JE, Pang CHN (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63

    Article  Google Scholar 

  • Huss VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192

    Article  CAS  PubMed  Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism, vol 3. Academic Press, New York, pp 21–132

    Chapter  Google Scholar 

  • Kelly KL, Judd DB (1976) Color. Universal language and dictionary of names (National Bureau of Standards special publication 440). Washington, DC: US Department of Commerce

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367

    Article  CAS  Google Scholar 

  • Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–179

    Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) CLUSTALW and CLUSTALX version 2. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 34:435–444

    Article  Google Scholar 

  • Li WJ, Xu P, Zhang LP, Tang SK, Cui XL, Mao PH, Xu LH, Schumann P, Stackebrandt E, Jiang CL (2003) Streptomonospora alba sp. nov., a novel halophilic actinomycete, and emended description of the genus Streptomonospora Cui et al. 2001. Int J Syst Evol Microbiol 53:1421–1425

    Article  CAS  PubMed  Google Scholar 

  • Marchal N, Bourdon JL, Richard CL (1987) Les milieux de culture pour l’isolement et l’identification biochimique des bactéries. Doin Press, Paris

    Google Scholar 

  • Meklat A, Sabaou N, Zitouni A, Mathieu F, Lebrihi A (2011) Halophilic actinomycetes in Saharan soils of Algeria: isolation, taxonomy and antagonistic properties. Appl Environ Microbiol 77:6710–6714

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Minnikin DE, O’Donnell AG (1984) Actinomycete envelope lipid and peptidoglycan composition. In: Goodfellow M, Mordarski M, Williams ST (eds) The biology of the actinomycetes. Academic Press, London, pp 337–388

    Google Scholar 

  • Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117

    Article  CAS  Google Scholar 

  • Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092

    Article  CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Technical note 101. Microbial ID, Newark

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Waksman SA (1961) Classification, identification, and descriptions of genera and species. In: The actinomycetes, vol. 2. Baltimore, Williams & Wilkins, pp 331–332

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) International committee on systematic bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Zhang DF, Pan HQ, He J, Zhang XM, Zhang YG, Klenk HP, Hu JC, Li WJ (2013) Description of Streptomonospora sediminis sp. nov. and Streptomonospora nanhaiensis sp. nov., and reclassification of Nocardiopsis arabia (Hozzein and Goodfellow, 2008) as Streptomonospora arabica comb. nov. and emended description of the genus Streptomonospora. Int J Syst Bacteriol 63:4447–4455

    Article  CAS  Google Scholar 

  • Zhi XY, Li WJ, Stackebrandt E (2009) An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to gratefully acknowledge the technical assistance of Gabriele Pötter and Bettina Sträubler (both at DSMZ).

Author information

Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria

    Atika Meklat, Noureddine Bouras, Amar Riba, Abdelghani Zitouni & Nasserdine Sabaou

  2. Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université Saâd Dahleb, Blida, Algeria

    Atika Meklat

  3. Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), Université de Toulouse, INPT-ENSAT, 1 Avenue de l’Agrobiopôle, BP 32607, 31326, Castanet-Tolosan, Auzeville Tolosane, France

    Florence Mathieu

  4. HZI – Helmholtz Centre for Infection Research, 38124, Brunswick, Germany

    Manfred Rohde

  5. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Peter Schumann, Cathrin Spröer & Hans-Peter Klenk

Authors
  1. Atika Meklat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Noureddine Bouras
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Amar Riba
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abdelghani Zitouni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Florence Mathieu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Manfred Rohde
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Peter Schumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Cathrin Spröer
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hans-Peter Klenk
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Nasserdine Sabaou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nasserdine Sabaou.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 3879 kb)

Supplementary material 2 (PPTX 417 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Meklat, A., Bouras, N., Riba, A. et al. Streptomonospora algeriensis sp. nov., a halophilic actinomycete isolated from soil in Algeria. Antonie van Leeuwenhoek 106, 287–292 (2014). https://doi.org/10.1007/s10482-014-0195-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0195-3

Keywords

Search

Navigation