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Thermochemical pretreatment enhanced bioconversion of elephant grass (Pennisetum purpureum): insight on the production of sugars and lignin

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Abstract

Pennisetum purpureum, commonly known as elephant grass, possesses an immense ability to be recognized as bioenergy crop due to its high carbohydrate content and ample availability in the northeastern states of India. In the present study, an alkaline treatment was conducted using NaOH of different concentrations (1%, 2% and 3% (w/v)) at varying reaction periods of 30, 60 and 90 min with an aspiration of maximum delignification and an enhanced recovery of carbohydrates from biomass. A maximum delignification of 81.0% (w/w) was obtained when the biomass was pretreated with 2% (w/v) of NaOH for 60 min while in the presence of 3% (w/v) of NaOH, the highest cellulose recovery of 72.3% (w/w) was accounted at 90 min of reaction time. During saccharification of biomass, a maximum 18.4 g/L of glucose was produced using 20 filter paper unit of commercial cellulase after 48 h of enzymatic reaction with a cellulose conversion of more than 46%. Furthermore, product inhibition study showed the significant inhibitory effect on enzymatic production of sugars even in the presence of 2 g/L glucose and xylose in the reaction system. The severity of alkaline treatment of the untreated and treated biomass was evaluated using different physiochemical analyses. Moreover, the extracted lignin obtained after acid precipitation of alkaline filtrate was characterised using UV-Visible spectrophotometer. Finally, two-way ANOVA revealed a significant variation in the production of glucose and xylose from biomass even when glucose was added at a fixed concentration (P value = 0.00044 << 0.05) into the reaction system. On the other hand, xylose shows significant inhibition in glucose and xylose production from biomass at both of a particular (P value = 9.05 × 10−7 << 0.05) and different (P value = 0.0078 << 0.05) concentrations of extraneous addition.

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References

  1. Ascencio JJ, Chandel AK, Philippini RR, Silva SS (2019) Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. Biomass Conv Bioref. https://doi.org/10.1007/s13399-019-00547-6

  2. Guarneros-Flores J et al (2019) Maximization of fermentable sugar production from sweet sorghum bagasse (dry and wet bases) using response surface methodology (RSM). Biomass Convers Biorefin 9:633–639

    Article  Google Scholar 

  3. Wang S et al (2017) Ultrasound assisted alkaline pretreatment to enhance enzymatic saccharification of grass clipping. Energy Convers Manag 149:409–415

    Article  Google Scholar 

  4. Xie X et al (2018) A sustainable and effective potassium hydroxide pretreatment of wheat straw for the production of fermentable sugars. Bioresour Technol Rep 3:169–176

    Article  Google Scholar 

  5. Ajjan FN et al (2016) High performance PEDOT/lignin biopolymer composites for electrochemical supercapacitors. J Mater Chem A 4:1838–1847

    Article  Google Scholar 

  6. Song K, Chu Q, Hu J, Bu Q, Li F, Chen X, Shi A (2019) Two-stage alkali-oxygen pretreatment capable of improving biomass saccharification for bioethanol production and enabling lignin valorization via adsorbents for heavy metal ions under the biorefinery concept. Bioresour Technol 276:161–169

    Article  Google Scholar 

  7. Haldar D, Purkait MK (2020) Lignocellulosic conversion into value-added products: a review. Process Biochem 89:110–133

    Article  Google Scholar 

  8. Ji H et al (2018) Valorization of lignocellulosic biomass toward multipurpose fractionation: furfural, phenolic compounds, and ethanol. ACS Sustain Chem Eng 6:15306–15315

    Article  Google Scholar 

  9. Haldar D, Sen D, Gayen K (2018) Enzymatic hydrolysis of banana stems (Musa acuminata): optimization of process parameters and inhibition characterization. Int J Green Energy 15:406–413

    Article  Google Scholar 

  10. Basso V et al (2014) Different elephant grass (Pennisetum purpureum) accessions as substrates for enzyme production for the hydrolysis of lignocellulosic materials. Biomass Bioenergy 71:155–161

    Article  Google Scholar 

  11. Pensri B et al (2016) Potential of fermentable sugar production from Napier cv. Pakchong 1 grass residue as a substrate to produce bioethanol. Energy Procedia 89:428–436

    Article  Google Scholar 

  12. Phitsuwan P, Sakka K, Ratanakhanokchai K (2016) Structural changes and enzymatic response of Napier grass (Pennisetum purpureum) stem induced by alkaline pretreatment. Bioresour Technol 218:247–256

    Article  Google Scholar 

  13. Eliana C et al (2014) Effects of the pretreatment method on enzymatic hydrolysis and ethanol fermentability of the cellulosic fraction from elephant grass. Fuel 118:41–47

    Article  Google Scholar 

  14. Minmunin J, Limpitipanich P, Promwungkwa A (2015) Delignification of elephant grass for production of cellulosic intermediate. Energy Procedia 79:220–225

    Article  Google Scholar 

  15. Bensah EC, Kádár Z, Mensah MY (2019) Alkali and glycerol pretreatment of West African biomass for production of sugars and ethanol. Bioresour Technol Rep 6:123–130

    Article  Google Scholar 

  16. Segal L et al (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794

    Article  Google Scholar 

  17. Dil EA et al (2016) Application of artificial neural network and response surface methodology for the removal of crystal violet by zinc oxide nanorods loaded on activate carbon: kinetics and equilibrium study. J Taiwan Inst Chem Eng 59:210–220

    Article  Google Scholar 

  18. Roosta M, Ghaedi M, Daneshfar A, Darafarin S, Sahraei R, Purkait MK (2014) Simultaneous ultrasound-assisted removal of sunset yellow and erythrosine by ZnS:Ni nanoparticles loaded on activated carbon: optimization by central composite design. Ultrason Sonochem 21:1441–1450

    Article  Google Scholar 

  19. Wang Q et al (2015) Cell wall disruption in low temperature NaOH/urea solution and its potential application in lignocellulose pretreatment. Cellulose 22:3559–3568

    Article  Google Scholar 

  20. MacDonald DG et al (1983) Alkali treatment of corn stover to improve sugar production by enzymatic hydrolysis. Biotechnol Bioeng 25:2067–2076

    Article  Google Scholar 

  21. Sharma SK, Kalra KL, Grewal HS (2002) Enzymatic saccharification of pretreated sunflower stalks. Biomass Bioenergy 23:237–243

    Article  Google Scholar 

  22. Kaschuk JJ Santos DdeA, Frollini E, Canduri F, Porto ALM (2019) Influence of pH, temperature, and sisal pulp on the production of cellulases from Aspergillus sp. CBMAI 1198 and hydrolysis of cellulosic materials with different hemicelluloses content, crystallinity, and average molar mass. Biomass Conv Bioref. https://doi.org/10.1007/s13399-019-00440-2

  23. Kataria R, Ghosh S (2014) NaOH pretreatment and enzymatic hydrolysis of Saccharum spontaneum for reducing sugars production. Energ Source Part A 36:1028–1035

    Article  Google Scholar 

  24. Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne J (2007) A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol 98:3000–3011

    Article  Google Scholar 

  25. Lima MA et al (2013) Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production–part 1. Biotechnol Biofuels 6:75

    Article  Google Scholar 

  26. Chen XH et al (2014) Asparagus stem as a new lignocellulosic biomass feedstock for anaerobic digestion: increasing hydrolysis rate, methane production and biodegradability by alkaline pretreatment. B Bioresour Technol 164:78–85

    Article  Google Scholar 

  27. Xu J et al (2010) Sodium hydroxide pretreatment of switchgrass for ethanol production. Energy Fuel 24:2113–2119

    Article  Google Scholar 

  28. Sahoo D, Ummalyma SB, Okram AK, Sukumaran RK, George E, Pandey A (2017) Potential of Brachiaria mutica (Para grass) for bioethanol production from Loktak Lake. Bioresour Technol 242:133–138

    Article  Google Scholar 

  29. Paixao SM et al (2016) Sugarcane bagasse delignification with potassium hydroxide for enhanced enzymatic hydrolysis. RSC Adv 6:1042–1052

    Article  Google Scholar 

  30. McIntosh S, Vancov T (2011) Optimisation of dilute alkaline pretreatment for enzymatic saccharification of wheat straw. Biomass Bioenergy 35:3094–3103

    Article  Google Scholar 

  31. Hu Z, Wen Z (2008) Enhancing enzymatic digestibility of switchgrass by microwave-assisted alkali pretreatment. Biochem Eng J 38:369–378

    Article  Google Scholar 

  32. McIntosh S, Vancov T (2010) Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment. Bioresour Technol 101:6718–6727

    Article  Google Scholar 

  33. Yanez-S M et al (2014) Physicochemical characterization of ethanol organosolv lignin (EOL) from Eucalyptus globulus: effect of extraction conditions on the molecular structure. Polym Degrad Stab 110:184–194

    Article  Google Scholar 

  34. Binod P et al (2012) Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse. Renew Energy 37:109–116

    Article  Google Scholar 

  35. Phitsuwan P et al (2015) Structural features and enzymatic digestibility of Napier grass fibre treated with aqueous ammonia. J Ind Eng Chem 32:360–364

    Article  Google Scholar 

  36. Kristensen JB et al (2008) Cell-wall structural changes in wheat straw pretreated for bioethanol production. Biotechnol Biofuels 1:5

    Article  Google Scholar 

  37. Lu Q-l et al (2014) An investigation on the characteristics of cellulose nanocrystals from Pennisetum sinese. Biomass Bioenergy 70:267–272

    Article  Google Scholar 

  38. Scalbert A et al (1986) Comparison of wheat straw lignin preparations-I. Chemical and spectroscopic characterizations. Holzforschung 40:119–127

    Article  Google Scholar 

  39. Reddy KO, Maheswari CU, Reddy DJP, Rajulu AV (2009) Thermal properties of Napier grass fibers. Mater Lett 63:2390–2392

    Article  Google Scholar 

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Acknowledgements

Central Instrumental Facility (CIF) and Centre for the Environment of IIT Guwahati are acknowledged for providing the instrumental facility to perform physicochemical characteristics of the samples.

Funding

This work in financially supported by the MHRD (Ministry of Human Resource and Development; Government of India) through the institute.

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Authors and Affiliations

  1. Centre for the Environment, Indian Institute of Technology Guwahati, Assam, 781039, India

    Dibyajyoti Haldar

  2. Department of Chemical Engineering, IIT Guwahati, Assam, 781039, India

    Mihir Kumar Purkait

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  1. Dibyajyoti Haldar
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  2. Mihir Kumar Purkait
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Correspondence to Dibyajyoti Haldar or Mihir Kumar Purkait.

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Haldar, D., Purkait, M.K. Thermochemical pretreatment enhanced bioconversion of elephant grass (Pennisetum purpureum): insight on the production of sugars and lignin. Biomass Conv. Bioref. 12, 1125–1138 (2022). https://doi.org/10.1007/s13399-020-00689-y

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