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Influence of different chemical pretreatments of elephant grass (Pennisetum purpureum, Schum.) used as a substrate for cellulase and xylanase production in submerged cultivation

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Abstract

This study evaluated the potential use of elephant grass biomass, a highly productive species, for cellulase and xylanase production by the cellulolytic mutant Penicillium echinulatum 9A02S1 in submerged cultivation, using untreated biomass, biomass pretreated with different concentrations of NaOH, H2SO4 or NH4OH, or biomass pretreated with H2O at 121 °C. For filter paper activity, all cultivation carried out with pretreated elephant grass under the evaluated conditions showed superior activity when compared with the control (untreated elephant grass). The activities of endoglucanases and β-glucosidases were higher in the cultivation prepared from pretreated samples than the control made with cellulose (Celuflok®). Without pretreatment, elephant grass can be used for xylanase production, enabling similar activities to those obtained in the cultivation with cellulose, reducing the enzyme production cost. These results indicate that the pretreatment of elephant grass, especially when pretreated with H2SO4, may be used as a partial or total replacement for cellulose to cellulase production, and untreated elephant grass may be used for xylanase production.

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Acknowledgments

The authors are grateful for the financial and technical support from UCS, CNPq (310590/2009-4) and FAPERGS (10/1972-5).

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Authors and Affiliations

  1. Institute of Biotechnology, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, Caxias do Sul, RS, 95070-560, Brazil

    Daiane Menegol, Angélica Luisi Scholl, Aldo José Pinheiro Dillon & Marli Camassola

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  1. Daiane Menegol
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  2. Angélica Luisi Scholl
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  3. Aldo José Pinheiro Dillon
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  4. Marli Camassola
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Correspondence to Marli Camassola.

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Menegol, D., Scholl, A.L., Dillon, A.J.P. et al. Influence of different chemical pretreatments of elephant grass (Pennisetum purpureum, Schum.) used as a substrate for cellulase and xylanase production in submerged cultivation. Bioprocess Biosyst Eng 39, 1455–1464 (2016). https://doi.org/10.1007/s00449-016-1623-8

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  • DOI: https://doi.org/10.1007/s00449-016-1623-8

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