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Maximization of fermentable sugar production from sweet sorghum bagasse (dry and wet bases) using response surface methodology (RSM)

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Abstract

Sweet sorghum bagasse (SSB) is an agroindustrial waste with a high fiber composition (34–44% cellulose, 25–27% hemicellulose, and 18–20% lignin), widely used in the production of second-generation biofuels. In this study, a response surface design (Box-Behnken) was used to optimize the enzymatic hydrolysis process, using Cellic CTec 3 enzyme. Treatment time (24–72 h), enzyme concentration (5–7% w/w), and liquid solid ratio (LSR) (5–9 v/w) were evaluated to maximize glucose production. The process was optimized both on a dry basis (solar radiation drying after each stage) and on a wet basis (continuous mode). The optimized conditions obtained in the enzymatic hydrolysis on a dry basis were 5% w/w enzyme concentration, 51-h treatment time, and 5: 1 v/w liquid solid ratio (LSR), obtaining 125.2 g/L glucose. For wet basis, the following results were obtained: 5% w/w enzyme concentration, 48-h treatment time, and 5: 1 v/w LSR, obtaining 148.64 g/L glucose. Optimized SSB on a wet basis showed an increase of 18.71% in glucose compared to that obtained on a dry basis, a reduction of 5.88% in process time, and elimination of 96-h solar drying, all of which favor continuous glucose production.

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Acknowledgements

The authors acknowledge the critical reading of Patricia Margaret Hayward-Jones, M.Sc., and Dulce María Barradas-Dermitz, M.Sc.

Funding

Economic support was provided by the National Council of Science and Technology and the Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food, Mexico (CONACyT-SAGARPA, Project 291143).

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Authors and Affiliations

  1. Graduate Studies and Research Division, National Institute of Technology of Mexico, Orizaba Institute of Technology, Oriente 9 852, C.P. 94320 Orizaba, Veracruz, Mexico

    Javier Guarneros-Flores, José Luis Morales-Martínez & Leticia López-Zamora

  2. Food Research and Development Unit, National Institute of Technology of Mexico, Veracruz Institute of Technology, Calz. M.A. de Quevedo 2779, C.P, 91860, Veracruz, Mexico

    María Guadalupe Aguilar-Uscanga

Authors
  1. Javier Guarneros-Flores
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  2. María Guadalupe Aguilar-Uscanga
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Correspondence to Leticia López-Zamora.

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Guarneros-Flores, J., Aguilar-Uscanga, M.G., Morales-Martínez, J.L. et al. Maximization of fermentable sugar production from sweet sorghum bagasse (dry and wet bases) using response surface methodology (RSM). Biomass Conv. Bioref. 9, 633–639 (2019). https://doi.org/10.1007/s13399-018-00366-1

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