Abstract
The rapid pace of economic development has resulted in the release of several polycyclic aromatic hydrocarbons (PAHs) into the environment. Microbial degradation using white-rot fungi is a promising method for the removal of PAHs from the environment. In the present study, biodegradation of recalcitrant PAH by a white-rot fungus, Trametes maxima IIPLC-32, was investigated using pyrene. The pyrene concentration decreased by 79.80%, 65.37%, and 56.37% within 16 days from the initial levels of 10 mg L−1, 25 mg L−1, and 50 mg L−1, respectively. Gas chromatographic–mass spectrometric identification of prominent metabolites 1-hydroxypyrene, 2-methyl-1-naphthyl acetic acid, di-n-butyl phthalate, and diethyl phthalate helped in determining the pyrene degradation pathway. The presence of 81 extracellular proteins was revealed by secretome analysis. The identified proteins up-regulated in response to pyrene degradation were classified into detoxification proteins (6.12%), redox proteins (6.12%), stress proteins (4.08%), metabolic-related proteins (26.53%), translation and transcriptional proteins (49%), catalytic proteins (49%), and other proteins (8.16%). Knowledge of secretome analysis in pyrene degradation helped to understand the degradation mechanism of pyrene. Also, the study suggests that T. maxima IIPLC-32 has the potential to be used in the bioremediation of PAH contaminated aquatic environment.
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Change history
27 February 2023
A Correction to this paper has been published: https://doi.org/10.1007/s11356-023-26148-5
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Acknowledgements
The authors are highly thankful to Dr. Vijay Tak, Scientist at Defence Research and Development Establishment (Gwalior, India), and Mr. Saurabh Nagpal, Agilent Technologies (Gurugram, India) for LC-MSn analysis.
Funding
This study was supported by the Council of Scientific and Industrial Research (CSIR), New Delhi, India, under the project OLP-1094; INSPIRE program, Department of Science and Technology (New Delhi, India) and Academy of Scientific and Innovative Research (Ghaziabad, India) provided fellowship.
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AI contributed to conceptualization, writing—original draft, and formal analysis; SKS contributed to writing—reviewing and editing, methodology, and validation; BPV and DT performed formal analysis; AR was involved in conceptualization, visualization, and funding acquisition; PKK contributed to conceptualization, resources, investigation, writing—reviewing and editing, and supervision. All the authors read and approved the final manuscript.
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Imam, A., Suman, S.K., Vempatapu, B.P. et al. Pyrene remediation by Trametes maxima: an insight into secretome response and degradation pathway. Environ Sci Pollut Res 29, 44135–44147 (2022). https://doi.org/10.1007/s11356-022-18888-7
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DOI: https://doi.org/10.1007/s11356-022-18888-7