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Effects of partial replacement of fish meal by yeast hydrolysate on antioxidant capability, intestinal morphology, and inflammation-related gene expression of juvenile Jian carp (Cyprinus carpio var. Jian)

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Abstract

This study aimed to evaluate the effects of fish meal (FM) replacement by yeast hydrolysate (YH) on liver antioxidant capability, intestinal morphology, and inflammation-related genes of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 600 fish (average initial weight 19.44 ± 0.06 g) were randomly selected and divided into five groups. Five isonitrogenous and isocaloric diets replacing FM by YH 0% (YH0), 1% (YH1), 3% (YH3), 5% (YH5), and 7% (YH7) were formulated. Each diet was tested in four replicates for 10 weeks. The results have shown that, compared to the control group (YH0), liver total superoxide dismutase (t-SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione (GSH) activities of fish fed YH1 and YH3 diets were significantly higher (P < 0.05). Liver malondialdehyde (MDA) concentration significantly increased as supplementation levels of YH increased from 1 to 7% (P < 0.05). Moreover, intestinal microvillus length of juvenile Jian carp fed YH diets were significantly higher than that of fish fed the control diet (P < 0.05). In proximal intestine, the relative expression levels of inflammation-related genes (ALP, IL-1β, and TNF-α) in YH7 were significantly higher than that in the control group (P < 0.05). However, in midintestine, the expression levels of these genes in YH3 were significantly lower compared to the control group (P < 0.05). The results of this study indicated that dietary replacement of FM by 3%YH could improve antioxidant capability and intestinal microvillus morphology, as well as enhance the non-specific immunity of juvenile Jian carp.

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Funding

This research was funded by the earmarked fund for China Agriculture Research System (CARS-45-14), the National Natural Science Foundation of China (31502178) and the Fundamental Research Funds for the Central Universities (KJQN201612).

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Authors and Affiliations

  1. Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province, 210095, People’s Republic of China

    Xiang-Yang Yuan, Guang-Zhen Jiang, Cong-Cong Wang, Kenneth Prudence Abasubong & Wen-Bin Liu

  2. Guangdong Hinabiotech CO., Ltd, Guangzhou, 511400, People’s Republic of China

    Qing Zou & Yu-Yan Zhou

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  1. Xiang-Yang Yuan
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  2. Guang-Zhen Jiang
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Yuan, XY., Jiang, GZ., Wang, CC. et al. Effects of partial replacement of fish meal by yeast hydrolysate on antioxidant capability, intestinal morphology, and inflammation-related gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). Fish Physiol Biochem 45, 187–197 (2019). https://doi.org/10.1007/s10695-018-0552-7

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