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0D/2D heteronanostructure–integrated bimetallic CoCu-ZIF nanosheets and MXene-derived carbon dots for impedimetric cytosensing of melanoma B16-F10 cells

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Abstract

A novel heterogeneous architecture has been constructed integrating two-dimensional (2D) bimetallic CoCu–zeolite imidazole framework (CoCu-ZIF) and zero-dimensional (0D) Ti3C2Tx MXene-derived carbon dots (CDs) (represented by CoCu-ZIF@CDs). The prepared CoCu-ZIF@CDs were further explored as sensitive layer for anchoring B16-F10 cell–targeted aptamer strands and detecting B16-F10 cells from the biological environment. Basic characterization showed that CDs were homogeneously embedded within CoCu-ZIF NSs owing to their π-π stacking interaction, leading to outstanding fluorescence performance of the 0D/2D CoCu-ZIF@CD nanohybrid. As such, the CoCu-ZIF@CD-based cytosensor was applied to detect living B16-F10 cells through electrochemical techniques and cell imaging. Compared with CoCu-ZIF- and CD-based cytosensors, the constructed CoCu-ZIF@CD-based one showed superior sensing performance, with an extremely low limit of detection (LOD) of 33 cells∙mL−1 and a wide range of suspension concentration of 1 × 102–1 × 105 cells∙mL−1 B16-F10 cells. The developed cytosensor also demonstrated excellent detection performance, including cell imaging properties, good selectivity, high stability, and good reproducibility. By anchoring other probe molecules, the constructed CoCu-ZIF@CD-based biosensor can be extensively explored for early diagnosis of other analytes, thereby widening the applications of porous organic frameworks in biosensing and biomedical fields.

Graphical abstract

A novel sensing system for melanoma B16-F10 cells based on a novel CoCu-ZIF@CD nanohybrid has been developed. The CoCu-ZIF@CDs-based cytosensor displayed an extremely low limit of detection (LOD) of 33 cells∙mL−1 within the wide range of B16-F10 cell concentration from 1 × 102 to 1 × 105 cells∙mL−1, accompanying with cell imaging properties, good selectivity, high stability, and well reproducibility

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Funding

This work was supported by the Programs for the National Natural Science Foundation of China (NSFC: Account Nos. U1604127, 81601082, and 81371363) and China Postdoctoral Science Foundation (2019M660175).

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Authors and Affiliations

  1. Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450052, People’s Republic of China

    Yang Liu, Jiangnan Li & Nan Zhou

  2. School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Shunjiang Huang, Minghua Wang, Changbao Wang, Bin Hu & Zhihong Zhang

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  1. Yang Liu
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  2. Shunjiang Huang
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  3. Jiangnan Li
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  4. Minghua Wang
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  5. Changbao Wang
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  6. Bin Hu
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  7. Nan Zhou
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  8. Zhihong Zhang
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Correspondence to Nan Zhou or Zhihong Zhang.

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Liu, Y., Huang, S., Li, J. et al. 0D/2D heteronanostructure–integrated bimetallic CoCu-ZIF nanosheets and MXene-derived carbon dots for impedimetric cytosensing of melanoma B16-F10 cells. Microchim Acta 188, 69 (2021). https://doi.org/10.1007/s00604-021-04726-z

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