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Molecular characterization of Italian rice cultivars

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Abstract

Random amplified polymorphic DNA (RAPD) is an efficient tool to detect DNA polymorphisms and to distinguish different clones and cultivars. We have applied the RAPD technique to distinguish, starting from a single rice seed, among some Italian rice cultivars from market and from National Institute for research on food and nutrition, Italy. We also estimated the genomic similarity among the rice cultivars object of this study taking advantage of the amplification patterns. Despite the low genomic variability among Italian rice cultivars, RAPD technique allowed us to distinguish among them. This indicates that RAPD can be used routinely to verify the identity of Italian rice cultivars in order to prevent fraudulent commercial activity.

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Abbreviations

CTAB:

Cetyltrimethylammonium bromide

MVSP:

Multivariate statistical package for windows

NL:

Nei and Li’s similarity index

PTB:

Phenacyl tiazolium bromide

RAPD:

Random amplified polymorphic DNA

UPGMA:

Unweighted pair group method using arithmetic average

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Acknowledgments

This study is dedicated to the memory of Prof. Antonino Cascino, who passed away on April 6th 2008. We are greatly indebted to Mrs. M.R. Cipollaro and Mrs O. Matarazzo for their administrative assistance, and to Jean Ann Gilder (Scientific Communication sas) for text editing and to Prof. Robert G. Milne (Institute of Plant Virology, CNR, Torino, Italy) for his constructive criticism in revising the manuscript. This research was supported by the National Institute for research on food and nutrition (INRAN) Progetto qualità alimentare to A.C. (2006) and partially by Fondo Integrativo Speciale per la Ricerca (FISR), Tracciabilità genetica per la qualità e sicurezza della filiera della carne e quantizzazione di Organismi Geneticamente Modificati (2005–2009) to M.C.

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Authors and Affiliations

  1. Section of Biotechnology and Molecular Biology Antonino Cascino, Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 16, 80138, Naples, Italy

    Alessandra Cirillo, Stefania Del Gaudio, Giovanni Di Bernardo, Umberto Galderisi, Antonino Cascino & Marilena Cipollaro

Authors
  1. Alessandra Cirillo
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  2. Stefania Del Gaudio
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  3. Giovanni Di Bernardo
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  4. Umberto Galderisi
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  5. Antonino Cascino
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  6. Marilena Cipollaro
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Corresponding author

Correspondence to Marilena Cipollaro.

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Cirillo, A., Del Gaudio, S., Di Bernardo, G. et al. Molecular characterization of Italian rice cultivars. Eur Food Res Technol 228, 875–881 (2009). https://doi.org/10.1007/s00217-008-1000-1

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  • DOI: https://doi.org/10.1007/s00217-008-1000-1

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