Abstract
Rust and powdery mildew diseases of wheat are among the oldest and most harmful diseases confining the yield of wheat worldwide. Development of resistant wheat cultivars with durable or race-nonspecific resistance genes is the main objective for many breeding programs. One of these genes, Lr34/Yr18/Pm38, was found to confer partial and durable resistance against rust pathogens as well as powdery mildew. This multiple disease resistant locus was recently found to comprise a single gene, which encodes an adenosine triphosphate-binding cassette transporter (ABC-transporter) of the pleiotropic drug resistance (PDR) subfamily. Differences in the alleles of resistant and susceptible cultivars enabled to develop six allele-specific markers. In this study, 46 different Turkish bread wheat cultivars were screened using these specific markers, which indicated that 13 cultivars carry the resistance allele of that gene. To compare this molecular finding with phenotypic observations, a field trial with artificial inoculation of Puccinia recondita was performed. Interestingly, all cultivars having the resistance allele were found to be susceptible. Although Lr34 has mediated resistance against leaf rust for over 40 years and has been used globally as an effective component of rust resistance in many breeding programs, this adult plant resistance is ineffective in the studied Turkish bread wheat cultivars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akin B, Zencirci N & Özseven I, 2008. Field resistance of wheat (Triticum aestivum L.) genotypes from different countries to leaf rust (Puccinia triticina). Turk J Agric For 32, 479–486.
Bossolini E, Krattinger SG & Keller B, 2006. Development of SSR markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor Appl Genet 113, 1049–1062.
Braun HJ, Zencirci N, Altay F, Atli A, Muzaffer A, Esser V, Kambertay M & Payne TS, 2001. Turkish wheat pool. In: Bonjean AP & Angus WJ (Ed.) 2001: The world wheat book: A history of wheat breeding. Intercept, London. 851–879.
Dusunceli L, Getin F, Albustan S & Beniwal SPS, 1996. Occurrence and impact of wheat stripe rust (Puccinia striiformis) In Turkey in 1994/95 crop season. 9th European and Mediterranean Cereal Rusts and Powdery Mildews Conference. Netherlands. 309.
Doyle JJ & Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin 19, 11–15.
Yildirim-Ersoy F, Ridout CJ & Akkaya MS, 2011. Detection of physically interacting proteins with the CC and NB-ARC domains of a putative yellow rust resistance protein, Yr10, in wheat. J Plant Dis Protect 118, 119–126.
Incirli A & Akkaya MS, 2001. Assessment of genetic relationships in durum wheat cultivars using AFLP markers. Genet Resour Crop Ev 48, 233–238.
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL & Keller B, 2009. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323, 1360–1363.
Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino JH, Obonnaya FC, Raman H, Orford S, Bariana HS & Lagudah ES, 2008. Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 4, 1841–1852.
Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS & Spielmeyer W, 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114, 21–30.
Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL & Keller B, 2009. Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119, 889–898.
McIntosh RA, Wellings CR & Park RF, 1995. Wheat Rusts: An Atlas of Resistance Genes. Kluwer Academic Publishers, Dordrecht, the Netherlands.
Morgounov A, Tufan HA, Sharma R, Akin B, Bagci A, Braun HJ, Kaya Y, Keser M, Payne TS, Sonder K & McIntosh R, 2012. Global incidence of wheat rusts and powder y mildew during 1969-2010 and durability of resistance of winter wheat variety Bezostaya 1. Eur J Plant Pathol 132, 323–340.
Peterson RF, Campbell AB & Hannah AE, 1948. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can J Res Sect C 26, 496–500.
Singh RP, 1992. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82, 835–838.
Singh RP, 1993. Genetic association of gene Bdv1 for tolerance to barley yellow dwarf virus with genes Lr34 and Yr18 for adult plant resistance to rusts in bread wheat. Plant Dis 77, 1103–1106.
Singh RP, Huerta-Espino J & Rajaram S, 2000. Achieving near immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phyt Hung 35, 133–139.
Singh RP & Huerta-Espino J, 2003. Effect of leaf rust resistance gene Lr34 on components of slow rusting at seven growth stages in wheat. Euphytica 129, 371–376.
Singh RP, Huerta-Espino J, Bhavani S, Herrera-Foessel SA, Singh D, Singh PK, Velu G, Mason RE, Jin Y & Njau P, 2010. Non-race specific resistance to rusts in CIMMYT spring wheats: breeding advances. In: Borlaug Global Rust Initiative 2010 Technical Workshop. St. Petersburg, Russian Federation.
Spielmeyer W, McIntosh RA, Kolmer J & Lagudah ES, 2005. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust co-segregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111, 731–735.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yildirim, K., Boylu, B., Atici, E. et al. In Turkish wheat cultivars the resistance allele of LR34 is ineffective against leaf rust. J Plant Dis Prot 119, 135–141 (2012). https://doi.org/10.1007/BF03356432
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03356432