Abstract
Leaf rust (caused by Puccinia triticina Eriks—Pt) occurs regularly in the Czech Republic and can inflict considerable yield losses on wheat, particularly in the southeastern part of the country in warm and dry years. Knowledge of resistance genes (Lr) is useful in the breeding, particularly when combined with data on virulence in the rust population. Seeds of 56 winter wheat cultivars registered in the Czech Republic were obtained from the Central Institute for Supervising and Testing in Agriculture. All cultivars were tested by molecular markers to postulate the presence of Lr1, Lr10, Lr24, Lr26, Lr28, Lr34, Lr37 and Lr68. In total, 39 cultivars were also tested for resistance to leaf rust in the field trials. The Lr37 gene had the highest proportion, occurring in 24 tested varieties. The most effective was the Lr24 gene, which was carried in various combinations with other Lr genes by cultivars Athlon, Futurum, Sheriff, Hyfi, Asory, Campesino, Gordian. Also the gene Lr28 was mostly highly effective.
Similar content being viewed by others
References
Bariana HS, Miah H, Brown GN, Willey N, Lehmensiek A (2007) Molecular mapping of durable rust resistance in wheat and its implication in breeding. Wheat production in stressed environments. Springer, Dordrecht, pp 723–728
Bartoš P, Šíp V, Chrpová J et al (2002) Achievements and prospects of wheat breeding for disease resistance. Czech J Genet Plant Breed 38(1):16–28. https://doi.org/10.17221/6107-cjgpb
Cherukuri DP, Gupta SK, Charpe A et al (2005) Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica 143:19–26. https://doi.org/10.1007/s10681-005-1680-6
Goyeau H, Lannou C (2011) Specific resistance to leaf rust expressed at the seedling stage in cultivars grown in France from 1983 to 2007. Euphytica 178:45–62. https://doi.org/10.1007/s10681-010-0261-5
Gultyaeva EI, Kanyuka IA, Alpateva NV et al (2009) Molecular approaches in identifying leaf rust resistance genes in Russian wheat varieties. Russ Agric Sci 35:316–319. https://doi.org/10.3103/s1068367409050085
Gupta SK, Charpe A, Koul S et al (2006) Development and validation of SCAR markers co-segregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica 150:233–240. https://doi.org/10.1007/s10681-006-9113-8
Hanzalová A, Dumalasová V, Zelba O (2021) Virulence in the Puccinia triticina population in the Czech Republic and resistance genes in registered cultivars 1966–2019. Euphytica 217:4. https://doi.org/10.1007/s10681-020-02733-4
Helguera M, Khan IA, Kolmer J et al (2003) PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci 43:1839–1847. https://doi.org/10.2135/cropsci2003.1839
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L et al (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486. https://doi.org/10.1007/s00122-012-1802-1
Horáková V, Dvořáčková O (2020) Seznam doporučených odrůd (2020) Pšenice ozimá, pšenice jarní, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý. Brno. Available from https://eagri.cz/public/web/file/650948/Obilniny_2020.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O (2019) Seznam doporučených odrůd 2019: pšenice ozimá, pšenice jarní, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý. Brno. Available from https://eagri.cz/public/web/file/625530/obilniny_2019.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O (2018) Seznam doporučených odrůd 2018: pšenice ozimá, pšenice jarní, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý. Brno. Available from https://eagri.cz/public/web/file/585839/Obilniny_2018.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O (2017) Seznam doporučených odrůd 2017: pšenice ozimá, pšenice jarní, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý. Brno. Available from https://eagri.cz/public/web/file/534436/Obilniny_2017.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O (2016) Seznam doporučených odrůd 2016: pšenice ozimá, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý. Brno. Available from https://eagri.cz/public/web/file/464985/Obilniny_2016.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O, Mezlík T (2015) Seznam doporučených odrůd 2015: pšenice ozimá, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý, hrách polní. Brno. Available from https://eagri.cz/public/web/file/407038/Obilniny_2015.pdf. Accessed 14 Dec 2021
Horáková V, Dvořáčková O, Mezlík T (2014) Seznam doporučených odrůd 2014: pšenice ozimá, ječmen jarní, ječmen ozimý, tritikale ozimé, oves setý pluchatý, hrách polní. Brno. Available from https://eagri.cz/public/web/file/313338/Obilniny_a_luskoviny_2014.pdf. Accessed 14 Dec 2021
Lagudah ES, McFadden H, Singh RP et al (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30. https://doi.org/10.1007/s00122-006-0406-z
Mago R, Spielmeyer W, Lawrence G, Lagudah E, Ellis J, Pryor A (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet 104(8):1317–1324. https://doi.org/10.1007/s00122-002-0879-3
McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379. https://doi.org/10.1146/annurev.phyto.40.120501.101443
Miedaner T, Korzun V (2012) Marker-assisted selection for disease resistance in wheat and barley breeding. Phytopathology 102(6):560–566. https://doi.org/10.1094/PHYTO-05-11-0157
Pathan AK, Park RF (2006) Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars: leaf rust resistance in European wheat cultivars. Euphytica 149(3):327–342. https://doi.org/10.1007/s10681-005-9081-4
Prins R, Groenewald JZ, Marais GF, Snape JW, Koebner RMD (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103(4):618–624. https://doi.org/10.1007/PL00002918
Qiu JW, Schürch AC, Yahiaoui N (2007) Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theor Appl Genet 115:159–168. https://doi.org/10.1007/s00122-007-0551-z
Riaz A, Periyannan S, Aitken E, Hickey L (2016) A rapid phenotyping method for adult plant resistance to leaf rust in wheat. Plant Methods 12:17. https://doi.org/10.1186/s13007-016-0117-7
Roelfs AP, Singh RP, Saari EE (1992) Rust disease of wheat: concepts and methods of management. CIMMYT, Mexico D.F, p 81
Serfling A, Krämer I, Lind V, Schliephake E, Ordon F (2011) Diagnostic value of molecular markers for Lr genes and characterization of leaf rust resistance of German winter wheat cultivars with regard to the stability of vertical resistance. Eur J Plant Pathol 130:559–575. https://doi.org/10.1007/s10658-011-9778-2
Stakman EC, Stewart PM, Loegering WO (1962) Identification of physiologic races of Puccinia graminis var. tritici. United States Department of Agriculture, Washington, DC
Zelba O, Hanzalová A, Dumalasová V, Viehmannová I (2022) Analyzing wheat cultivars grown in Czech Republic for eight stem rust resistance genes. Eur J Plant Pathol 162:221–230. https://doi.org/10.1007/S10658-021-02397-3
Acknowledgements
Authors thank Ing. Pavel Bartoš, DrSc. for cooperation in preparation of the manuscript.
Funding
This research was funded by the Ministry of Agriculture of the Czech Republic, Project No. MZE RO0418, Project NAZV QK1910041 and by the Internal Grant Agency of the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, IGA (Project No. 20213114), and funded by European Union, Horizon 2020, Grant agreement No 773311.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare that they have no conflicts of interest.
Consent to participate
Not applicable.
Ethics approval
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Hanzalová, A., Zelba, O. Leaf rust (Puccinia triticina Eriks) resistance genes in wheat cultivars registered in the Czech Republic. J Plant Dis Prot 129, 1087–1093 (2022). https://doi.org/10.1007/s41348-022-00625-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s41348-022-00625-4