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Narrow Genetic Variability in Cicer arietinum L. as Revealed by RFLP Analysis

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Abstract

A subgenomic library constructed from small Pstl restriction fragments (0.4 to 2.0 kb) yielded 83.18% low-copy clones. Using 17 random genomic and 5 heterologous probes in 65 probe-enzyme combinations, Restriction Fragment Length Polymorphism (RFLP) for nuclear DNA was studied in five desi and five kabuli type chickpea cultivars. Only two clones revealed polymorphism in the cultivars tested. No polymorphism in chickpea varieties was detected with four Random Amplified Polymorphic DNA (RAPD) markers studied. However, some degree of polymorphism between C. arietinum and its wild relative C. reticulatum was detected. The RFLP analysis of chloroplast and mitochondrial genomes showed no polymorphism.

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  1. Biotechnology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India

    S. M. Udupa, Anuradha Sharma, R. P. Sharma & R. A. Pai

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  1. S. M. Udupa
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  2. Anuradha Sharma
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  3. R. P. Sharma
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  4. R. A. Pai
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Udupa, S.M., Sharma, A., Sharma, R.P. et al. Narrow Genetic Variability in Cicer arietinum L. as Revealed by RFLP Analysis. J. Plant Biochem. Biotechnol. 2, 83–86 (1993). https://doi.org/10.1007/BF03262930

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  • DOI: https://doi.org/10.1007/BF03262930

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