Skip to main content
SpringerLink
Log in

Gas-chromatographic determination of levamisole in human plasma-normalization and reliability of the method

  • Published:
European Journal of Drug Metabolism and Pharmacokinetics Aims and scope Submit manuscript

Summary

Levamisole has been used by many authors as an immunostimulant agent but the relationship between oral doses, plasma level and the therapeutic effects were not known.

For such a study it was necessary to establish an accurate and reproducible method of measurement. We describe a modifi-cation of the gas chromatographic method of Wynants et al. (1975). The normalization of the extraction process leads to greater reliability and accuracy (4 ng±7.6 %). Reproducibility was established using a series of 30 determinations from standard solutions and plasmas supplemented with Levamisole. The relative standard deviation in K evaluation was varied between 0.9 and 1.8%, 3.7 and 6.1% for standard solutions and plasmas respectively.

Consequently this method was adopted for this pharmacokinetic study which was carried out on healthy and cancer patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Renoux G., Renoux M. (1971): Effet immunostimulant d’un imidothiazole dans l’immunisation des souris contre l’infection par Brucella abortus. CR. Acad. Sci.272, 349.

    CAS  Google Scholar 

  2. Cazin J.C., Lesieur D., Cazin M., Lesieur I., Luyckx M., Devulder B., Plouvier B., Rousseau F. (1978): Propos sur le lévamisole. Bull. Soc. Pharm. (Lille)34 (1), 17–29.

    CAS  Google Scholar 

  3. Rousseau F. (1978): Dosage du Lévamisole dans le plasma par Chromatographie en phase gazeuse. Diplôme d’Etudes Approfondies. Fac Pharm. Lille.

    Google Scholar 

  4. Wynants J., Woestenborghs R., Heykants J. (October 1975). The gas chromatographic determination of lévamisole in body fluids and tissues. In: Janssen Research Products Information Service, Section 11: Biological research reports.

Download references

Author information

Authors and Affiliations

  1. Service de Médecine Générale et Néphrologie B, Hôpital Calmette, Lille, France

    Frederique Rousseau

  2. Laboratoires de Chimie, Faculté de Pharmacie, Rue du Professeur Laguesse, Lille, France

    Jean-Marie Haguenoer, Daniel Lesieur & Andre-Paul Gamot

Authors
  1. Frederique Rousseau
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean-Marie Haguenoer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniel Lesieur
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andre-Paul Gamot
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rousseau, F., Haguenoer, JM., Lesieur, D. et al. Gas-chromatographic determination of levamisole in human plasma-normalization and reliability of the method. European Journal of Drug Metabolism and Pharmacokinetics 6, 281–288 (1981). https://doi.org/10.1007/BF03189527

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03189527

Key words

Search

Navigation