Abstract
A method is proposed for determining rhein (an active metabolite of diacerein) in human blood plasma by reversed-phase high-performance liquid chromatography with UV detection. The analyte was selectively extracted from blood plasma by liquid-liquid extraction with a 5% solution of tert-butyl methyl ether in ethyl acetate. The recovery of rhein is 68 ± 3%, LOQ 100 ng/mL. The stability of the analytes and linearity, selectivity, sensitivity, reproducibility, and accuracy of the developed method are evaluated. The pharmacokinetics of diacerein in the oral administration of drug formulations by 18 healthy volunteers is studied.
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Original Russian Text © I.S. Yaroshenko, A.Ya. Khaimenov, A.V. Grigoriev, A.A. Sidorova, 2014, published in Zhurnal Analiticheskoi Khimii, 2014, Vol. 69, No. 8, pp. 868–874.
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Yaroshenko, I.S., Khaimenov, A.Y., Grigoriev, A.V. et al. Determination of Rhein in blood plasma by HPLC with UV detection and its application to the study of bioequivalence. J Anal Chem 69, 793–799 (2014). https://doi.org/10.1134/S1061934814080127
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DOI: https://doi.org/10.1134/S1061934814080127