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Genetic diversity and evolutionary relationships ofOryza species with the B- and C-genomes as revealed by SSR markers

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Abstract

Genetic diversity and evolutionary relationships of 72 accessions representing six species with the B-, C-, and BC-genomes in the genusOryza were investigated by seven microsatellite markers. Of four diploid species,Oryza officinalis maintained the highest diversity (P=71.4%, He=0.565), followed by Oryza eichingeri (P=57.1%, He=0.376), Oryzapunctata (P=57.1%, He=0.272) and Oryza rhizomatis (P=42.9%, He=0.222). In comparison, a higher level of genetic diversity was revealed in the tetraploid (P=71.4%, He=0.461-0.637). UPGMA dendrograms based on genetic distance revealed an obvious genetic differentiation between Asian and African races ofO. eichingeri. Three BBCC species clustered with different accessions of the diploidO. punctata, suggestive of their multiple origins. The results inferred from the dendrogram suggested that diploid species,O. officinalis and AfricanO. eichingeri might be the C-genome donors for tetraploid species,Oryza minuta andO. punctata, respectively, while the C-genome ancestor ofOryza malampuzhaensis seemed to be eitherO. rhizomatis or the Sri LankanO. eichingeri species. The genetic relationship among the CC and BBCC species further indicated that the tetraploid species with the BC-genome have originated independently, at least three times in history. In addition, we have demonstrated successful cross-species amplification of seven rice SSR loci acrossOryza species with B-and C-genomes.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, 310029, Hangzhou, China

    Ying Bao & De Yuan Hong

  2. College of Life Science, Qufu Normal University, Qufu 273165, Shandong, China

    Ying Bao

  3. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    Hai Fei Zhou, De Yuan Hong & Song Ge

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  1. Ying Bao
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Correspondence to Song Ge.

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Bao, Y., Zhou, H.F., Hong, D.Y. et al. Genetic diversity and evolutionary relationships ofOryza species with the B- and C-genomes as revealed by SSR markers. J. Plant Biol. 49, 339–347 (2006). https://doi.org/10.1007/BF03178809

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