Skip to main content
SpringerLink
Log in

Saturation transfer EPR studies of slow rotational motion in membranes

  • Published:
Applied Magnetic Resonance Aims and scope Submit manuscript

Abstract

Saturation transfer spectroscopy is a nonlinear electron paramagnetic resonance method for detecting rotational diffusion of nitroxide spin labels on the microsecond timescale or slower. Various results on spin-labeled biological membranes are reviewed here. These studies include those on lipid motions in gel-phase phospholipid bilayers, on the dynamics of lipid-protein interactions, and on the rotational diffusion of spin-labeled transmembrane proteins. Certain methodological aspects are also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Marsh D., Watts A. in: Lipid-Protein Interactions (Jost P.C., Griffith O.H., eds.), vol. 2 pp. 53–126. New York: Wiley-Interscience 1982.

    Google Scholar 

  2. Esmann M., Sar P.C., Hideg K., Marsh D.: Anal. Biochem.213, 336–348 (1993)

    Article  Google Scholar 

  3. Esmann M., Hankovszky H.O., Hideg K., Pedersen J.A., Marsh D.: Anal. Biochem.189, 274–282 (1990)

    Article  Google Scholar 

  4. Marchetto R., Schreier S., Nakaie C.R.: J. Am. Chem. Soc.115, 11042–11043 (1993)

    Article  Google Scholar 

  5. Esmann M., Hankovszky H.O., Hideg K., Marsh D.: Biochim. Biophys. Acta978, 209–215 (1989)

    Article  Google Scholar 

  6. Horváth L.I., Dux L., Hankovszky H.O., Hideg K., Marsh D.: Biophys. J.58, 231–241 (1990)

    Article  Google Scholar 

  7. Esmann M., Hideg K., Marsh D.: Biochemistry33, 3693–3697 (1994)

    Article  Google Scholar 

  8. Monaco V., Formaggio F., Crisma M., Toniolo C., Hanson P., Millhauser G.L.: Biopolymers50, 239–253 (1999)

    Article  Google Scholar 

  9. Marsh D.: J. Magn. Reson.180, 305–310 (2006)

    Article  ADS  Google Scholar 

  10. Cherry R.J., Godfrey R.E.: Biophys. J.36, 257–276 (1981)

    Article  ADS  Google Scholar 

  11. Jähnig F.: Eur. Biophys. J.14, 63–64 (1986)

    Article  Google Scholar 

  12. Thomas D.D., Dalton L.R., Hyde J.S.: J. Chem. Phys.65, 3006–3024 (1976)

    Article  ADS  Google Scholar 

  13. Marsh D., Horváth L.I.: J. Magn. Reson.99, 323–331 (1992)

    Google Scholar 

  14. Marsh D.: Appl. Magn. Reson.3, 53–65 (1992)

    Article  ADS  Google Scholar 

  15. Horváth L.I., Marsh D.: J. Magn. Reson.80, 314–317 (1988)

    Google Scholar 

  16. Robinson B.H., Dalton L.R.: J. Chem. Phys.72, 1312–1324 (1980)

    Article  ADS  Google Scholar 

  17. Beth A.H., Hustedt E.J. in: Biomedical EPR, part B (Eaton G.R., Eaton S.S., Berliner L.J., eds.). Biological Magnetic Resonance, vol. 24, pp. 369–407. New York: Kluwer 2005.

    Google Scholar 

  18. Beth A.H., Robinson B.H. in: Spin Labeling: Theory and Applications (Berliner L.J., Reuben J., eds.). Biological Magnetic Resonance, vol. 8, pp. 179–253. New York: Plenum 1989.

    Google Scholar 

  19. Fajer P., Marsh D.: J. Magn. Reson.51, 446–459 (1983)

    Google Scholar 

  20. Fajer P., Watts A., Marsh D.: Biophys. J.61, 879–891 (1992)

    Article  ADS  Google Scholar 

  21. Watts A., Marsh D.: Biochim. Biophys. Acta642, 231–241 (1981)

    Article  Google Scholar 

  22. Marsh D.: Biochemistry19, 1632–1637 (1980)

    Article  Google Scholar 

  23. Marsh D., Watts A.: Biochem. Biophys. Res. Commun.94, 130–137 (1980)

    Article  Google Scholar 

  24. Horváth L.I., Marsh D.: J. Magn. Reson.54, 363–373 (1983)

    Google Scholar 

  25. Marsh D., Horváth L.I.: J. Magn. Reson.97, 13–26 (1992)

    Google Scholar 

  26. Páli T., Bartucci R., Horváth L.I., Marsh D.: Biophys. J.64, 1781–1788 (1993)

    Article  Google Scholar 

  27. Páli T., Bartucci R., Horváth L.I., Marsh D.: Biophys. J.61, 1595–1602 (1992)

    Article  Google Scholar 

  28. Bartucci R., Páli T., Marsh D.: Biochemistry32, 274–281 (1993)

    Article  Google Scholar 

  29. Marsh D., Horváth L.I.: Biochim. Biophys. Acta1376, 267–296 (1998)

    Google Scholar 

  30. Horváth L.I., Brophy P.J., Marsh D.: Biophys. J.64, 622–631 (1993)

    Article  Google Scholar 

  31. Li G., Horváth L.I., Knowles P.F., Murphy D.J., Marsh D.: Biochim. Biophys. Acta987, 187–192 (1989)

    Article  Google Scholar 

  32. Kleinschmidt J.H., Powell G.L., Marsh D.: Biochemistry37, 11579–11585 (1998)

    Article  Google Scholar 

  33. Páli T., Kleinschmidt J.H., Powell G.J., Marsh D.: Biochemistry39, 2355–2361 (2000)

    Article  Google Scholar 

  34. Marsh D. in: Spin Labeling: Theory and Applications (Berliner, L.J., Reuben J., eds.). Biological Magnetic Resonance vol. 8, pp., 255–303. New York: Plenum 1989.

    Google Scholar 

  35. Horváth L.I., Brophy P.J., Marsh D.: Biochim. Biophys. Acta1147, 277–280 (1993)

    Article  Google Scholar 

  36. Brophy P.J., Horváth L.I., Marsh D.: Biochemistry23, 860–865 (1984)

    Article  Google Scholar 

  37. Arora A., Esmann M., Marsh D.: Biochemistry38, 10084–10091 (1999)

    Article  Google Scholar 

  38. Livshits V.A., Páli T., Marsh D.: J. Magn. Reson.133, 79–91 (1998)

    Article  ADS  Google Scholar 

  39. Livshits V.A., Dzikovski B.G., Marsh D.: J. Magn. Reson.162, 429–442 (2003)

    Article  ADS  Google Scholar 

  40. Hustedt E.J., Beth A.H.: Biophys. J.69, 1409–1423 (1995)

    Article  ADS  Google Scholar 

  41. Hebert H., Purhonen P., Vorum H., Thomsen K., Maunsbach A.B.: J. Mol. Biol.314, 479–494 (2001)

    Article  Google Scholar 

  42. Esmann M., Horváth L.I., Marsh D.: Biochemistry26, 8675–8683 (1987)

    Article  Google Scholar 

  43. Nury H., Dahout-Gonzalez C., Trézéguet V., Lauquin G., Brandolin G., Pebay-Peyroula E.: FEBS Lett.579, 6031–6036 (2005)

    Article  Google Scholar 

  44. Horváth L.I., Munding A., Beyer K., Klingenberg M., Marsh D.: Biochemistry28, 407–414 (1989)

    Article  Google Scholar 

  45. Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.: Acta Crystallogr. D56, 529–535 (2000)

    Article  Google Scholar 

  46. Fajer P., Knowles P.F., Marsh D.: Biochemistry28, 5634–5643 (1989)

    Article  Google Scholar 

  47. Toyoshima C., Nakasako M., Nomura N., Ogawa H.: Nature405, 647–655 (2000)

    Article  ADS  Google Scholar 

  48. de Jongh H.H.J., Hemminga M.A., Marsh D.: Biochim. Biophys. Acta1024, 82–88 (1990)

    Article  Google Scholar 

  49. Teller D.C., Okada T., Behnke C.A., Palczewski K., Stenkamp R.E.: Biochemistry40, 7761–7772 (2001)

    Article  Google Scholar 

  50. Ryba N.J.P., Marsh D.: Biochemistry31, 7511–7518 (1992)

    Article  Google Scholar 

  51. Abramson J., Smirnova I., Kasho V., Verner G., Kaback H.R., Iwata S.: Science,301, 610–615 (2003)

    Article  ADS  Google Scholar 

  52. Marsh D., Henderson P.J.F.: Biochim. Biophys. Acta,1510, 464–473 (2001)

    Article  Google Scholar 

  53. Páli T., Finbow M.E., Holzenburg A., Findlay J.B.C., Marsh D.: Biochemistry34, 9211–9218 (1995)

    Article  Google Scholar 

  54. Páli T., Whyteside G., Dixon N., Kee T.P., Ball S., Harrison M.A., Findlay J.B.C., Finbow M.E., Marsh D.: Biochemistry43, 12297–12305 (2004)

    Article  Google Scholar 

  55. Páli T., Finbow M.E., Marsh D.: Biochemistry38, 14311–14319 (1999)

    Article  Google Scholar 

  56. Fox Jr. R.O., Richards F.M.: Nature300, 325–330 (1982)

    Article  ADS  Google Scholar 

  57. Marsh D., Jost M., Peggion C., Toniolo C.: Biophys. J. (2007) in press.

  58. Marsh D.: Eur. Biophys. J.26, 203–208 (1997)

    Article  Google Scholar 

  59. Knowles P.F., Watts A., Marsh D.: Biochemistry18, 4480–4487 (1979)

    Article  Google Scholar 

  60. Páli T., Bashtovyy D., Marsh D.: Protein Sci.15, 1153–1161 (2006)

    Article  Google Scholar 

  61. Powell G.L., Knowles P.F., Marsh D.: Biochemistry29, 5127–5132 (1990)

    Article  Google Scholar 

  62. Cone R.A.: Nature236, 39–43 (1972)

    Google Scholar 

  63. Downer N.W.: Biophys. J.47, 285–293 (1985)

    Article  ADS  Google Scholar 

  64. Fotiadis D., Liang Y., Filipek S., Saperstein D.A., Engel A., Palczewski K.: Nature421, 127–128 (2003)

    Article  ADS  Google Scholar 

  65. Nagle J.F., Tristram-Nagle S.: Biochim. Biophys. Acta1469, 159–195 (2000)

    Google Scholar 

  66. Lomize M.A., Lomize A.L., Pogozheva I., Mosberg H.I.: Bioinformatics22, 623–625 (2006)

    Article  Google Scholar 

  67. Ryba N.J.P., Hoon M.A., Findlay J.B.C., Saibil H.R., Wilkinson J.R., Heimburg T., Marsh D.: Biochemistry32, 3298–3305 (1993)

    Article  Google Scholar 

  68. Venien-Bryan C., Davies A., Langmack K., Baverstock J., Watts A., Marsh D., Saibil H.: FEBS Lett.359, 45–49 (1995)

    Article  Google Scholar 

  69. Marsh D.: Handbook of Lipid Bilayers. Boca Raton, Fla.: CRC Press 1990.

    Google Scholar 

  70. Esmann M., Karlish S.J.D., Sottrup-Jensen L., Marsh D.: Biochemistry33, 8044–8050 (1994)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Spektroskopie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany

    D. Marsh

Authors
  1. D. Marsh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. Marsh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marsh, D. Saturation transfer EPR studies of slow rotational motion in membranes. Appl. Magn. Reson. 31, 387–410 (2007). https://doi.org/10.1007/BF03166592

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03166592

Keywords

Search

Navigation