Abstract
This chapter addresses the determination of protein–lipid selectivity, here described as the preference of a protein for having a specific type of lipid in its vicinity, from Förster resonance energy transfer methodologies. These allow a quantification of the effect, that is, the determination of the biasing in distribution of the lipid under study around the protein, as compared to its bulk membrane distribution, with advantages over established approaches that have been used for the same purpose, such as electron spin resonance spectroscopy. The experiment can be carried out with steady-state instrumentation, the formalisms are described in detail, and the model can be applied to a membrane protein of any size.
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Acknowledgments
This work was partially supported by the Portuguese “Fundação para a Ciência e a Tecnologia” (FCT) through projects FAPESP/20107/2014, SAICTPAC/0019/2015, 007630 UID/QUI/00313/2013 and PT2020_PTDC_DTP-FTO_2784_2014, co-funded by COMPETE2020-UE and national funds. F.F. acknowledges an FCT researcher grant (IF/00386/2015).
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Loura, L.M.S., Prieto, M., Fernandes, F. (2019). Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity. In: Kleinschmidt, J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9512-7_16
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DOI: https://doi.org/10.1007/978-1-4939-9512-7_16
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