Abstract
CHO mutants, resistant to over 100-fold of a normally toxic level of extracellular cadmium have been used to examine the mutually antagonistic effect of Cd and Zn on their uptake. Cadmium uptake in these mutants is only 7–10% that of the parental cells. Zinc uptake in these mutants is equal to or greater than that in the wild-type cells. Results of kinetic studies on uptake indicated that the two metals interact by competitive inhibition. TheK m andK i values for Cd and/or Zn were different in some of the mutants and indicate multiple carriers may be involved in the transport of these metals. The reduction in Cd uptake and concomitant increase in Zn uptake contribute to the increased Cd resistance in these mutants.
Similar content being viewed by others
References
K. Schwarz and J. Spallholz,Fed. Proc. 35, 255 (1976).
M. Anke, A. Hennig, B. Groppel, M. Partschefeld, and M. Grun,Proceedings of the 3rd International Symposium on Trace Element Metabolism in Man and Animals (Tema 3), M. Kirchgessner, ed., 1978, pp. 540.
P. C. Huang, B. Smith, P. Bohdan, and A. Corrigan,Biol. Trace Element Res. 2, 211 (1980).
B. S. Kingsley and J. M. Frazier,Am. J. Physiol. 236, C139 (1979).
J. M. Frazier and B. S. Kingsley,Toxicol. Appl. Pharmacol. 38, 583 (1976).
C. P. Cox,Mol. Pharmacol. 4, 510 (1968).
N. H. Stacey and C. D. Klaassen,Toxicol. Appl. Pharmacol. 55, 448 (1980).
F. Haffner and H. E. Rugstad,Acta Pharmacol. Toxicol. 38, 525 (1976).
M. L. Failla and R. J. Cousins,Biochim. Biophys. Acta 538, 435 (1978).
M. D. Enger, L. T. Ferzoco, R. A. Tobey, and C. E. Hildebrand,J. Toxicol. Env. Health,7, 675 (1981).
C. E. Hildebrand, M. D. Enger, and R. A. Tobey,Biol. Trace Element Res. 2, 235 (1980).
M. L. Failla, R. J. Cousins, and M. J. Mascenik,Biochim. Biophys. Acta 583, 63 (1979).
M. M. Wintrobe, G. R. Lee, D. R. Baggs, T. C. Bithell, J. W. Athens and J. Foerster,Clinical Hematology, 7th ed., Lea and Febiger, Philadelphia, 1974.
K. D. Neame and T. G. Richards,Elementary Kinetics of Membrane Carrier Transport, Blackwell Scientific Publications, Oxford, England, 1972.
R. G. Blasberg, “Brain Barrier Systems,” inProgress in Brain Research, A. Lajtha and D. H. Ford, eds.,29, 245 (1968).
S. Silver, P. Johnseine, E. Whitney, and D. Clark,J. Bacteriol. 110, 186 (1972).
R. G. Duggleby,Anal. Biochem. 110, 19 (1981).
M. D. Enger, C. E. Hildebrand, M. Jones, and H. L. Barrington, inDevelopmental Toxicology of Energy-Related Pollutants Proc. 17th Ann. Hanford Biol. Symp., D. D. Mahlum, M.R. Sikov, P. L. Hackett, and F. D. Andrew, eds., (1978), p. 37.
E. C. Foulkes,J. Environ. Path. Tox. 3, 471 (1980).
C. J. Bowmer and W. E. Lindup,Biochim. Biophys. Acta 624, 260 (1980).
C. E. Hildebrand and L. S. Cram,Proc. Soc. Exp. Biol. Med. 161, 438 (1979).
M. D. Enger, L. B. Rall and C. E. Hildebrand,Nucleic Acids Res. 7, 271 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Corrigan, A.J., Huang, P.C. Cellular uptake of cadmium and zinc. Biol Trace Elem Res 3, 197–216 (1981). https://doi.org/10.1007/BF02990117
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02990117