Abstract
The influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, pH) was determined on biofilm formation by 51S. maltophilia clinical isolates. The strains produced more biofilm at 32 °C than at 37 or 18 °C. Aerobic and 6 % CO2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. Biofilm production was not affected by staticvs. agitated culture conditions. Biofilm production at pH 7.5 and 8.5 was comparable but significantly higher than at pH 5.5. The capacity of individual strains to form biofilm and thus contribute to the severity of some diseases is influenced by host traits and environmental conditions at the site of infection, and play an important role in the pathogenesis of biomaterial-related disease caused byS. maltophilia.
Similar content being viewed by others
References
de Abreu Vidipò L., de Andrade Marques E., Puchelle E., Plotkowski M.C.:Stenotrophomonas maltophilia interaction with human epithelial respiratory cellsin vitro.Microbiol.Immunol. 45, 563–569 (2001).
Biswas S.K., Chaffin W.L.: Anaerobic growth ofCandida albicans does not support biofilm formation under similar conditions used for aerobic biofilm.Curr.Microbiol. 51, 100–104 (2005).
Černohorská L., Votava M.: Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.Folia Microbiol. 49, 75–78 (2004).
Costerton J.W., Stewart P.S., Greenberg E.P.: Bacterial biofilms: a common cause of persistence infections.Science 284, 1318–1322 (1999).
Dancer S.J.: Mopping up hospital infection.J.Hosp.Infect. 43, 85–100 (1999).
Denton M., Kerr K.G.: Microbiological and clinical aspects of infection associated withStenotrophomonas maltophilia.Clin.Microbiol.Rev. 11, 57–80 (1998).
Di Bonaventura G., Spedicato I., d’Antonio D., Robuffo I., Piccolomini R.: Biofilm formation byStenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime.Antimicrob.Agents Chemother. 48, 151–160 (2004).
Donaldson S.H., Boucher R.C.: Update on pathogenesis of cystic fibrosis lung disease.Curr.Opin.Pulm.Med. 9, 486–491 (2003).
Guiney D.G.: Regulation of bacterial virulence gene expression by the host environment.J.Clin.Invest. 99, 565–569 (1997).
Harjai K., Khandwaha R.K., Mittal R., Yadav V., Gupta V., Sharma S.: Effect of pH on production of virulence factors by biofilm cells ofPseudomonas aeruginosa.Folia Microbiol. 50, 99–102 (2005).
Jucker B.A., Harms H., Zehnder A.J.: Adherence of the positively charged bacteriumStenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon.J.Bacteriol. 178, 5472–5479 (1996).
Kellum J.A.: Determinants of blood pH in health and disease.Crit.Care 4, 6–14 (2000).
Oie S., Kamiya A.: Microbial contamination of antiseptics and disinfectants.Am.J.Infect.Control 24, 389–395 (1996).
Růžička F., Holá V., Votava M., Tejkalová R., Horvát R., Heroldová M., Woznicová V.: Biofilm detection and the clinical significance ofStaphylococcus epidermidis isolates.Folia Microbiol. 49, 596–600 (2004).
Senol E., Desjardin J., Stark P.C., Barefoot L., Snydman D.R.: Attributable mortality toStenotrophomonas maltophilia bacteremia.Clin.Infect.Dis. 34, 1653–1656 (2002).
Squier C., Yu V.L., Stout J.E.: Waterborne nosocomial infections.Curr.Infect.Dis.Rep. 6, 490–496 (2000).
Stepanović S., Vuković D., Ježek P., Pavlović M., Švabić-Vlahović M.: Influence of dynamic conditions on biofilm formation by staphylococci.Eur.J.Clin.Microbiol.Infect.Dis. 20, 502–504 (2001).
Stepanović S., Ćirković I., Mijać V., Svabić-Vlahović M.: Influence of the incubation temperature, atmosphere and dynamic conditions on biofilm formation bySalmonella spp.J.Food Microbiol. 20, 339–343 (2003a).
Stepanović S., Djukić N., Djordjević V., Djukić S.: Influence of the incubation atmosphere on the production of biofilm by staphylococci.Clin.Microbiol.Infectol. 9, 955–958 (2003b).
Svabić-Vlahović M., Dakić I., Vuković D., Stepanović S.: Biofilm production byStaphylococcus epidermidis at 37 and 32 °C. Abstr. P926,13th Eur. Congr. Clin. Microbiol. Infect. Dis., Glasgow (UK) 2003.
Van Couwenberghe C., Cohen S.: Analysis of epidemic and endemic isolates ofXanthomonas maltophilia by contour clamped homogeneous electric field gel electrophoresis.Infect.Control Hosp.Epidemiol. 15, 691–696 (1994).
Walencka E., Sadowska B., Różalska S., Hryniewicz W., Różalska B.:Staphylococcus aureus biofilm as a target for single or repeated doses of oxacillin, vancomycin, linezolid and/or lysostaphin.Folia Microbiol. 51, 381–386 (2006).
Woodard L.M., Bielkie A.R., Eisses J.F., Ketchum P.A.: Occurrence of nitrate reductase and molybdopterin inXanthomonas maltophilia.Appl.Environ.Microbiol. 56, 3766–3771 (1990).
Worlitzsch D., Tarran R., Ulrich M., Schwab U., Cekici A., Meyer K.C., Birrer, P., Bellon G., Berger J.: Effect of reduced mucus oxygen concentration in airwayPseudomonas infections of cystic fibrosis patients.J.Clin.Invest. 109, 317–325 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was in part supported by the following grants of theIstituto di Ricovero e Cura a Carattere Scientifico (IRCCS) (“Don Gnocchi” — 1 % Sanità; 2003),Programmi di Ricerca di Rilevante Interesse Nazionale (PRIN) (2005), andItalian Cystic Fibrosis Research Foundation (2005).
Rights and permissions
About this article
Cite this article
Di Bonaventura, G., Stepanović, S., Picciani, C. et al. Effect of environmental factors on biofilm formation by clinicalStenotrophomonas maltophilia isolates. Folia Microbiol 52, 86–90 (2007). https://doi.org/10.1007/BF02932144
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02932144