Abstract
The structural gene coding for YompC has been identified in the genome of a pathogenic strain ofYersinia enterocolitica O:9, and was subsequently cloned and sequenced. Detailed alignment of the deduced amino acid sequence showed that YompC is a member of the OmpC porin family with the highest degree of homology toKlebsiella pneumoniae. The mutant lacking YompC porin was constructed by insertional inactivation of theyompC gene which resulted from the integration of suicide vector at theyompC locus. In intact cells ofY. enterocolitica, loss of the YompC protein reduced the outer membrane permeability for β-lactam antibiotics and tetracycline and resulted in a 2–5-fold increase in resistance to these compounds, depending on their chemical properties. Mutation in theompR regulatory gene resulted in the loss of both YompC and YompF porins, which led to a greater increase of resistance to antibiotics, as compared with the YompC mutant strain. Moreover, the binding assay with HEp-2 cells suggests that YompC may play a role in the adhesion properties ofY. enterocolitica strains.
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Abbreviations
- Chl:
-
chloramphenicol
- Kan:
-
kanamycin
- Nal:
-
nalidixic acid
- NB:
-
nutrient broth
- OM:
-
outer membrane(s)
- PAGE:
-
polyacrylamide gel electrophoresis
- PBS:
-
phosphate-buffered saline
- Y.ent. :
-
Yersinia enterocolitica
References
Armand-Lefevre L., Leflon-Guibout V., Bredin J., Barguellil F., Amor A., Peges J.M., Nicolas-Chanoine M.H.: Imipenem resistance inSalmonella enterica serovar Wien related to porin loss and CMY-4 β-lactamase production.Antimicrob.Agents Chemother.47, 1165–1168 (2003).
Benz R., Bauer K.: Permeation of hydrophilic molecules through the outer membrane of Gram-negative bacteria.Eur.J.Biochem.176, 1–19 (1988).
Bernardini M.L., Sanna M.G., Fontaine A., Sansonetti P.J.: OmpC is involved in invasion of epithelial cells byShigella flexneri.Infect.Immun.61, 3625–3635 (1993).
Blanco L.P., Toro C.S., Romero J.M., Santiviago C.A., Mora G.C.:Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes.Microbiol.Immunol.41, 999–1003 (1997).
Bottone E.J.:Yersinia enterocolitica: the charisma continues.Clin.Microbiol.Rev.10, 257–276 (1997).
Brubaker R.R.: Factors promoting acute and chronic diseases caused by yersiniae.Clin.Microbiol.Rev.4, 309–324 (1991).
Brzostek K., Hrebenda J., Benz R., Boos W.: The OmpC protein ofYersinia enterocolitica: purification and properties.Res.Microbiol.140, 599–614 (1989).
Brzostek K., Raczkowska A., Zasada A.: The osmotic regulator OmpR is involved in the response ofYersinia enterocolitica O:9 to environmental stresses and survival within macrophages.FEMS Microbiol.Lett.228, 265–271 (2003).
Chevalier J., Pages J.M., Mallea M.:In vivo modification of porin activity conferring antibiotic resistance toEnterobacter aerogenes.Biochem.Biophys.Res.Commun.266, 248–251 (1999).
Cornelis G.R., Boland A., Boyd A.P., Geuijen C., Iriarte M., Neyt C., Sory M.P., Stainier I.: The virulence plasmid ofYersinia, an antihost genome.Microbiol.Mol.Biol.Rev.62, 1315–1352 (1998).
De Lorenzo V., Timmis K.N.: Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposon.Meth.Enzymol.235, 386–405 (1994).
El Tahir Y., Skurnik M.: YadA, the multifacetedYersinia adhesion.Internat.J.Med.Microbiol.291, 209–218 (2001).
Figurski D.H., Helinski D.R.: Replication of an origin containing derivative of plasmid RK2 dependent on a plasmid function provided intrans.Proc.Nat.Acad.Sci.USA75, 1648–1652 (1979).
Galdiero F., Tufano M.A., Sommese L., Folgore A., Tedesco F.: Activation of complement system by porins extracted fromSalmonella typhimurium.Infect.Immun.46, 559–563 (1984).
Galdiero F., Sommese L., Scrafogliero P., Galdiero M.: Biological activities — lethality, Shwartzman reaction and pyrogenicity ofSalmonella typhimurium porins.Microbiol.Pathog.16, 111–119 (1995).
Hanahan D.: Studies on transformation ofEscherichia coli with plasmids.J.Mol.Biol.166, 557–580 (1983).
Hernandez-Alles S., Alberti S., Alvares D., Domenech-Samnches A., Martinez-Martinez L., Tomas J.M., Benedi V.J.: Porin expression in clinical isolates ofKlebsiella pneumoniae.Microbiology.145, 673–679 (1999).
Iriarte M., Vanooteghen J.C., Delor I., Diaz R., Knutton S., Cornelis G.R.: The Myf fibrillaeYersinia enterocolitica.Mol.Microbiol.9, 507–520 (1993).
Kinder S.A., Badger J.L., Bryant G.O., Pepe J.C., Miller V.L.: Cloning of theYenI restriction endonuclease and methyltransferase fromYersinia enterocolitica serotype O8 and construction of a transformable R-M+ mutant.Gene136, 271–275 (1993).
Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop II R.M., Peterson K.M.: Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.Gene166, 175–176 (1995).
Mallea M., Simonet V., Lee E.H., Gervier R., Collatz E., Gutmann L., Pages J.M.: Biological and immunological comparisons ofEnterobacter cloacae andEscherichia coli porins.FEMS Microbiol.Lett.129, 273–280 (1995).
Martinez M.B., Fickinger M., Higgins L., Krick T., Nelsestuen G.L.: Reduced outer membrane permeability ofEscherichia coli O157:H7: suggested role of modified outer membrane porins and theoretical function in resistance to antimicrobial agents.Biochemistry40, 11965–11974 (2001).
Martinez-Martinez L., Hernandez-Alles S., Alberti S., Tomas J.M., Benedi V.J., Jacoby G.A.:In vivo election of porin-deficient mutants ofKlebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins.Antimicrob.Agents Chemother.40, 342–348 (1996).
Miller V.L., Falkow S.: Evidence for two genetic loci inYersinia enterocolitica that can promote invasion of epithelial cells.Infect. Immun.56, 1242–1248 (1988).
Negm R.S., Pistole T.G.: The porin OmpC ofSalmonella typhimurium mediates adherence to macrophages.Can.J.Microbiol.45, 658–669 (1999).
Nikaido H.: Porins and specific channels of bacterial outer membranes.Mol.Microbiol.6, 435–442 (1992).
Nikaido H., Vaara M.: Molecular basis of bacterial outer membrane permeability.Microbiol.Rev.49, 1–32 (1985).
Nitzan Y., Orlovsky K., Pechatnikov I.: Characterization of porins isolated from the outer membrane ofSerratia liquefaciens.Curr.Microbiol.38, 71–79 (1999).
Pepe J., Miller V.L.:Yersinia enterocolitica invasin: a primary role in the initiation of infection.Proc.Nat.Acad.Sci.USA90, 6473–6477 (1993).
Sambrook J.E.F., Fritsch E.F., Maniatis T.:Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor (NY) 1997.
Seoane A., Garcia-Lobo J.M.: Cloning of chromosomal β-lactamase genes fromYersinia enterocolitica.J.Gen.Microbiol.137, 141–146 (1991).
Seoane A., Sanchez E., Garcia-Lobo J.M.: Tandem amplification of a 28-kilobase region fromYersinia enterocolitica chromosome containing theblaA gene.Animicrob.Agents Chemother.47, 682–688 (2003).
Simonet V.C., Basle A., Klose K.E., Delcour A.H.: TheVibrio cholerae porins OmpU and OmpT have distinct channel properties.J.Biol.Chem.278, 17539–17545 (2003).
Sonnevend Á., Czirók É., Pál T.:Yersinia Yop-specific IgA antibodies in Hungarian blood donors.Folia Microbiol.50, 269–272 (2005).
Tabaraie B., Sharma B.K., Sharma P.R., Sehgal R., Ganguly N.K.: Stimulation of macrophage oxygen free radical production and lymphocyte blastogenic response by immunization with porins.Microbiol.Immunol.38, 561–565 (1994).
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Brzostek, K., Raczkowska, A. The YompC protein ofYersinia enterocolitica: Molecular and physiological characterization. Folia Microbiol 52, 73–80 (2007). https://doi.org/10.1007/BF02932142
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DOI: https://doi.org/10.1007/BF02932142