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Intracellular colonization ofRhododendron andVaccinium roots byCenococcum geophilum, Geomyces pannorum andMeliniomyces variabilis

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Abstract

Fourin vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM)Cenococcum geophilum Fr. (strain CGE-4), saprotrophicGeomyces pannorum (Link)Sigler & Carmichael (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-formingMeliniomyces variabilis Hambleton & Sigler (MVA-1) in roots ofRhododendron andVaccinium. A typical ErM fungus,Rhizoscyphus ericae (Read)Zhuang & Korf (RER-1), was included for comparison. All fungal strains intracellularly colonized rootedVaccinium microcuttings: GPA-1 occasionally produced hyphal loops similar to ErM, MVA-1 and RER-1 exhibited a typical ErM colonization pattern. CGE-4 hyphae grew vigorously on and around newly formed roots and rarely penetrated turgescent rhizodermal cells forming intracellular loose loops. Rooting ofRhododendron sp. microcuttings was not promoted by any fungal strain except CGE-4, which also promoted the most vigorous growth ofRhododendron ponticum L. seedlings. The widespread EcM fungusC. geophilum has a potential to colonize non-EcM roots and support their development which may influence overall growth of ericaceous plants. As shown forG. pannorum, structures resembling ErM may be formed by fungi that are to date not regarded as ericoid mycorrhizal.

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Abbreviations

DSE:

dark septate endophytes

EcM:

ectomycorrhiza(l)

ErM:

ericoid mycorrhiza(l)

CGE:

Cenococcum geophilum

GPA:

Geomyces pannorum

MMN:

medium of Melin-Norkrans

MMR:

medium according to Mitchell and Read

MVA:

Meliniomyces variabilis

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Authors and Affiliations

  1. Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, 252 43, Průhonice, Czechia

    M. Vohník, M. Fendrych, J. Albrechtová & M. Vosátka

  2. Department of Plant Physiology, Faculty of Science, Charles University, 128 44, Prague, Czechia

    M. Vohník, M. Fendrych & J. Albrechtová

Authors
  1. M. Vohník
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  2. M. Fendrych
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  3. J. Albrechtová
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  4. M. Vosátka
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Correspondence to M. Vohník.

Additional information

This study was financed by theGrant Agency of the Charles University, Prague (project GAUK 144/2005/B-BIO/PrF), the COST E38.003 project 1P05OC081, and is a part of the research project AV 0Z 6005 0516 of theInstitute of Botany, Academy of Sciences of the Czech Republic. The first author was supported by theGrant Agency of the Czech Republic (project GACR 206/03/H137).

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Vohník, M., Fendrych, M., Albrechtová, J. et al. Intracellular colonization ofRhododendron andVaccinium roots byCenococcum geophilum, Geomyces pannorum andMeliniomyces variabilis . Folia Microbiol 52, 407–414 (2007). https://doi.org/10.1007/BF02932096

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  • DOI: https://doi.org/10.1007/BF02932096

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