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Electrophoretic investigation of ribonuclease in roots and leaves ofVicia faba L.

Elektroforetické sledování ribonukleázy v extraktech z různých růstových zon kořenu a z mladých a stárnoucích listůVicia faba L.

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Biologia Plantarum

Abstract

Isozyme patterns and specific activity of ribonuclease (ribonucleate pyridinenucleotido-2′-transferase, E. C. 2.7.7.16) were followed in the extracts of segments from three growth zones of the root and in extracts of young and senescent leaves ofVicia faba L. Electrophoreograms of extracts from all three investigated root zones were identical, in the electrophoreograms of extracts from senescent leaves however one new ribonuclease occurred which could not be detected in the electrophoreograms of extracts from young leaves. Extracts from senescent leaves had higher specific activity of ribonuclease than extracts from young leaves. Extracts from the enlargement zone of the root and those from the maturation zone had a three times higher specific activity of RNase than extracts from the division zone.

Abstract

V extraktech ze tří růstových zón kořenu a z mladých a stárnoucích listůVicia faba L. bylo sledováno isozymové složení a specifická aktivita ribonukleázy. Elektroforeogramy extraktů ze tří sledovaných zón kořenu byly identické, na elektroforeogramech extraktů ze stárnoucích listů se však ve srovnání s elektroforeogramy extraktů z mladých listů vyskytovala jedna isoribonukleáza navíc. Extrakty ze stárnoucích listů měly vyšší specifickou aktivitu ribonukleázy než extrakty z mladých listů. Extrakty z prodlužovací zóny kořenů a ze zóny s ukončeným dlouživým růstem měly třikrát vyšší specifickou aktivitu než extrakty z meristematické zóny.

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  1. Department of Plant Physiology, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    J. Sahulka

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  1. J. Sahulka
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Address: Flemingovo nám. 2. Praha-6, Czechoslovakia.

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Sahulka, J. Electrophoretic investigation of ribonuclease in roots and leaves ofVicia faba L.. Biol Plant 13, 243–246 (1971). https://doi.org/10.1007/BF02931016

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  • DOI: https://doi.org/10.1007/BF02931016

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