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Electrophoretic investigation of proteins in different root zones ofvicia faba L.

Elektroforetické sledování bílkovin v různých zonách kořene bobu koňskěho (Vicia faba L.)

Элктрофоретическое исследование исследование бслков в разных зопах корня конского боба (Vicia faba L.).

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Biologia Plantarum

Abstract

The differentiation of tissues is closely connected with the proteosynthesis. One can therfore assume that tissues with different types of cell growth (meristematic or elongation growth) and with different degrees of differentiation are different in their protein composition. In order to compare the protein composition of different plant organs, the method of disc electrophoresis on a polyacrylamide gel has been used by some authors. As compared with other methods used up to now, e. g. isolating proteins on DEAE cellulose or in Sephadex, this method does not need so much material and its resolution ability seems to be higher. It is also quicker and enables the study of several samples simultaneously. Its disadvantage is that proteins can be identified mainly by means of Rf and their quantity, measured from the intensity of staining of individual fractions in the gel, which may be misleading due to different sorption capacity of different proteins (FričFričová 1967). None the less, it is good for comparison of protein composition of individual parts of the plant body.

Different methods have been used to compare protein composition of individual growth zones in roots.Barsky,Ivanov andPushakova (1965) used luminiscence microscopy and found that in maize roots it is not possible to find substantial differences by this method.Morgan andReith (1954) arrived at similar conclusions. On the other hand,Steward et al. (1965) andMorris (1966) found qualitative differences in protein composition of different parts of pea roots using acrylamide electrophoresis. The results of the last named authors show considerable discrepancies in details, due perhaps to a different method of extraction (buffer, pH, purifying method).

We have used acrylamide gel electrophoresis for investigating proteins in precisely defined growth zones of theVicia faba root.

Abstract

Metodou akrylamidově elektroforesy nebyly zjištěny v meristematické, prodlužovací zoně a v zoně s ukončeným prodlužovacím růstem buněk podstatné kvalitativní rozdíly v bílkovinném složení. Ani kvantitativní rozdíly nebyly výrazné.

Je diskutována shoda většiny bίlkovinných frakcí kořene bobu koňského (Vicie faba) a hrachu (Pisum sativum).

Abstract

Элсктрофорезом в акриламиде не было найдсно пиких сушествснных различий в качственном состаяе белков между образцом из меристематической зоны удлинения и образцом из зопы с законченым удлинением клсток корняVicia faba L. Ра3личия в количсствеппом составе не были бостсвсрны. Обсуждается соответствие большинетва Rf белковых фраклий у корня копских бобов (Vicia faba L.) и гороха (pisum sativum)

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References

  • Adámková, A., Beneš, K.: Position and extent of the elongation zone in the root tip of the broad bean (Vicie feba L.) — Biol. Plant.8: 427–430, 1966.

    Google Scholar 

  • Beckmann, L., Scandalios, J. U., Brewbaker, J. L.: Catalase hybrid enzymes in maize. — Science146 (3648): 1174–1175, 1964.

    Article  Google Scholar 

  • Davis, B. J.: Disc electrophoresis II. Method and application to human serum proteins. — Ann. N. Y. Acad. Sci.121: 407–427, 1964.

    Article  Google Scholar 

  • Frič, F., Fričová, L.: Beitrag zur Auswertung von diskusförmigen Elektrophoreogrammen auf Polyakrylamidgel. — Biológia22: 22–29, 1967.

    PubMed  Google Scholar 

  • Kloz, J., Turková, V.: Legumin, vicillin and proteins similar to them in the seeds of some species of theViciaceae family. (A comparative serological study). — Biol. Plant.5: 29–40, 1963.

    Article  CAS  Google Scholar 

  • Morgan, C., Reith, W. S.: The composition and quantitative relation of protein and related fractions in developing root cells. — J. exp. Bot.5: 119–135, 1954.

    Article  CAS  Google Scholar 

  • Morris, R. O.: Change in pea root proteins associated with tissue differentiation: The effect of 2,4.dichlorphenoxyacetic acid. — Biochim. biophys. Acta127: 273–276, 1966.

    PubMed  CAS  Google Scholar 

  • Novacký, K., Macko, V., Hässler, J.: Poznámky k diskovej elektroforéze rastlinných bielkovin na akrylamidovom géli. [Notes to the disc electrophoresis of plant proteins on acrylamide gel]. — Biológia21: 64–68, 1966.

    PubMed  Google Scholar 

  • Safonov, V. I.: Investigation of proteins in the seeds of soya beans by the disc electrophoresis on the polyacrylamide gel. — Received for publ. in Biol. Plant. 1967.

  • Steward, F. C., Lyndon, R. F., Barber, J. T.: Acrylamide gel electrophoresis of soluble proteins: A study on pea seedlings in relation to development. — Amer. J. Bot.52: 155–164 1965.

    Article  CAS  Google Scholar 

  • Варсκий B. E.,Ивапов B. B.,Пушаκοва T. K.: Люминиcцентнο-микрοс кοпическοе иccледοвание распредеЛения и накοпленпя белκοв в κοрняхрастений — Йзв. AH CCCP, cep. биοл. 6: 916-921, 1965. [Barsky V. E.,Ivanov, V. B.,Pushakova, T. K.: Investigation of protein distribution and accumulation in plant roots by means of the luminiscence microscopy.]

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  1. Department of Plant Physiology Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo nám. 2, Praha 6 - Dejvice

    Věra Hadačová & J. Sahulka

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  1. Věra Hadačová
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  2. J. Sahulka
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Hadačová, V., Sahulka, J. Electrophoretic investigation of proteins in different root zones ofvicia faba L.. Biol Plant 9, 396–400 (1967). https://doi.org/10.1007/BF02929785

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