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Electrophoretic study on peroxidase, indoleacetic acid oxidase, and o-diphenol oxidase fractions in extracts from different growth zones ofvicia faba L. roots

Elektroforetická studie frakeí peroxidázy, oxidázy kyseliny indolyloctové a o-difenol oxidázy v extraktech z různých růstových zón kořenuVicia faba L.

  • Published:
Biologia Plantarum

Abstract

Using electrophoresis in acrylamide gel, fractions of peroxidase, indoleacetic acid oxidase, and o-diphenol oxidase were investigated in extracts from three growth zones ofVicia faba L. roots. Three peroxidase fractions (zones) moving towards the anode were revealed as well as four peroxidase fractions (zones) migrating towards the cathode. Three peroxidase fractions showed detectable indoleacetic acid oxidase activity. The o-diphenol oxidase activity was revealed in all peroxidase fractions moving towards the anode, in those moving towards the cathode the o-diphenol oxidase activity differred according to the substrate used. One fraction with both peroxidase and o-diphenol oxidase activity occurred only in electrophoreograms of extracts from the maturation zone; in this fraction no indoleacetic acid oxidase activity was demonstrable.

Abstract

Pomocí elektroforesy v akrylamidovém gelu byly sledovány frakce peroxidázy, oxidázy kyseliny indolyloctové a o-difenol oxidázy v extraktech ze tří růstových zón kořenuVicia faba L. Byly zjištěny tři peroxidázové frakce (zóny) pohybující se k anodě a čtyři peroxidázové frakce (zóny) pohybující se ke katodě. U tří peroxidázových frakcí byla zjištěna aktivita oxidázy kyseliny indolyloctové. O-difenol oxidázová aktivita byla zjištěna u všech peroxidázových frakcí pohybujících se k anodě, u frakcí pohybujících se ke katodě se o-difenol oxidázová aktivita lišila podle použitého substrátu. Jedna frakce s peroxidázovou i o-difenol oxidázovou aktivitou se vyskytovala pouze na elektroforeogramech extraktů ze zóny s ukončeným růstem; tato frakce neměla zjistitelnou aktivitu oxidázy kyseliny indolyloctové.

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  1. Department of Plant Physiology, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    J. Sahulka

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Address: Flemingovo nám. 2, Praha 6, Czechoslovakia.

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Sahulka, J. Electrophoretic study on peroxidase, indoleacetic acid oxidase, and o-diphenol oxidase fractions in extracts from different growth zones ofvicia faba L. roots. Biol Plant 12, 191–198 (1970). https://doi.org/10.1007/BF02920867

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  • DOI: https://doi.org/10.1007/BF02920867

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